60 male mice were divided randomly into four groups: 1) control group, 2) extremely low frequency-magnetic field group, 3) load aluminum group (200 mg aluminum/kg), and 4) "extremely low frequency-magnetic field + aluminum" group.
Exposure duration: 4 h daily, 6 days per week for 8 weeks
60 male mice were divided into four groups: 1) control group 2) extremely low frequency-magnetic field group 3) load aluminum group (200 mg aluminum/kg) 4) "extremely low frequency-magnetic field + aluminum" group
|magnetic flux density||2 mT||spatial average||measured||-||-|
After 8 weeks of treatment, the mice of the three experimental groups (groups 2-4) exhibited a memory impairment (escaping latency to the platform was prolonged and percentage of time spent in the platform quadrant was reduced in the Morris water maze task). Additionally, pathologic abnormalities including neuronal cell loss and overexpression of phosphorylated tau protein in the hippocampus and cerebral cortex was found in the three experimental groups. The data showed a statistically significant decrease in superoxide dismutase activity and an increase in the levels of malondialdehyde in the three experimental groups compared to the control group.
However, the treatment with magnetic field + aluminum induced no more damage than the magnetic field and aluminum alone (i.e. no synergistic effect).
In conclusion, both aluminum and extremely low frequency magnetic field could affect memory and pro-oxidative function in mice. However, there was no evidence of any association between extremely low frequency electromagnetic exposure with aluminum loading.