Medical/biological study (experimental study)

Extremely low frequency magnetic field induces oxidative stress in mouse cerebellum. med./bio.

By: Chu LY, Lee JH, Nam YS, Lee YJ, Park WH, Lee BC, Kim D, Chung YH, Jeong JH

Published in: Gen Physiol Biophys 2011; 30 (4): 415-421

Aim of study (acc. to author)

To study whether an extremely low frequency magnetic field induces lipid peroxidation and reactive oxygen species in mouse cerebellum.

Background/further details: 20 mice were divided into an exposure group and a sham exposure group (each group n=10).

Endpoint

effects on the neurological system: oxidative stress in mouse cerebellum

Exposure

- 50/60 Hz

Exposure	Parameters
Exposure 1: 60 Hz Exposure duration: three hours	magnetic flux density: 2.3 mT

Exposure 1

Main characteristics

Frequency	60 Hz
Type	magnetic field
Exposure duration	three hours

Exposure setup

Setup	mice were kept inside their cages during exposure
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	2.3 mT	-	-	-	-

Exposed system:

animal
mouse/BALB/c
whole body

Methods Endpoint/measurement parameters/methodology

effects on the neurological system: oxidative stress in mouse cerebellum: lipid peroxidation (malondialdehyde level; via thiobarbituric acid substances, spectrophotometry); antioxidant defense (enzyme activities of superoxide dismutase and glutathione peroxidase (spectrophotometry); levels of glutathione and ascorbic acid (photometry)); formation of reactive oxygen species (hydroxyl radical level (HPLC-ECD)

Investigated material:

tissue homogenates and supernatants

Investigated organ system:

brain/CNS

Time of investigation:

after exposure

Main outcome of study (acc. to author)

After exposure to the 60 Hz extremely low frequency magnetic field at 2.3 mT for three hours, there was a significant increase in malondialdehyde and hydroxyl radical levels. Exposure significantly induced concomitant increase in superoxide dismutase enzyme activity without alteration in glutathione peroxidase enzyme activity. While glutathione contents were not altered, ascorbic acid level was significantly decreased by extremely low frequency magnetic field exposure.
These results indicate that the extremely low frequency magnetic field may induce oxidative stress in mouse cerebellum.

Study character:

medical/biological study
experimental study
full/main study
Short Communication

Study funded by

Korea Research Foundation (KRF), Korea
Ministry of Education and Human Resources Development, Korea

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