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Medical/biological study (experimental study)

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation.

Published in: Adv Clin Exp Med 2015; 24 (3): 429-436

Aim of study (acc. to author)

The effects of exposure of rats to a 900 MHz electromagnetic field (GSM) on oxidative stress in the testes should be investigated and if an administration of melatonin prevents these effects.
Background/further details: Rats were divided into the following groups (n=21 each): 1) GSM exposure, 2) melatonin group (intraperitoneal injection of 2 mg/kg melatonin), 3) co-exposure (melatonin injection and GSM exposure), 4) control group (injection with saline).
After 20, 40 and 60 days, 7 rats from each group were sacrificed and analyzed, respectively.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 4 hours/day for 20, 40 or 60 days
Exposure 1
Main characteristics
Frequency 900 MHz
Type
Charakteristic
Exposure duration continuous for 4 hours/day for 20, 40 or 60 days
Additional info GSM signal
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber plastic cage without any metallic fittings
Setup 8 rats per cage (remark EMF-Portal: but 7 rats per cage were housed and sacrificed) were able to move freely; test phone was connected to a computer with an appropriate software module by a communication test set PC development kit
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 9.88 V/m minimum measured - -
electric field strength 18.36 V/m maximum measured - -
magnetic flux density 4.68 µT minimum measured - -
magnetic flux density 8.69 µT maximum measured - -
SAR 0.043 W/kg minimum estimated whole body -
SAR 0.135 W/kg maximum estimated whole body -
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After 20 days, only the lipid peroxidation and the enzyme activity of the alkaline DNase were significantly increased and the enzyme activity of the catalase was significantly reduced in the testes of GSM exposed mice (group 1) compared to the control group (group 4). After 40 and 60 days, the lipid peroxidation, protein oxidation and enzyme activities of xanthine oxidase, alkaline and acid DNase were significantly increased and the enzyme activity of the catalase was significantly reduced in GSM exposed mice compared to the control group.
Co-exposure to the GSM field and melatonin (group 3) significantly reduced lipid peroxidation, xanthine oxidase and acid DNAse enzyme activities compared to the GSM exposure group.
Mice injected with melatonin only (group 2) showed a significantly reduced acid DNAse enzyme activity after 20-60 days compared to the control group.
The authors conclude that exposure of rats to a 900 MHz electromagnetic field (GSM) could induce oxidative stress in the testes and that melatonin might attenuate the effect.
Study character:

Study funded by

  • Ministry of Science and Technological Development, Serbia

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