Due to a lack of financial resources, we unfortunately have to suspend the import of new articles regarding radio frequency and mobile communications as of now (November 27, 2017). If you have found the EMF-Portal useful and wish to support us, we will be very happy to accept your donation.

DONATION ACCOUNT: Uniklinik RWTH Aachen, IBAN: DE27 3905 0000 0013 0040 15, BIC: AACSDE33, Reference: GB-FM/380454/Arbm

More information →

Home
Medical/biological study (experimental study)

Therapeutic approaches of melatonin in microwave radiations-induced oxidative stress-mediated toxicity on male fertility pattern of Wistar rats.

Published in: Electromagn Biol Med 2014; 33 (2): 81-91

Aim of study (acc. to author)

The effects of exposure of rats to a 2.45 GHz electromagnetic field on oxidative stress and damage in the testes and the protective effect of melatonin should be investigated.
Background/further details: Rats were divided into 4 groups (n=6 each): 1) exposure to the electromagnetic field, 2) treatment with melatonin (daily intraperitoneal injection of 2 mg/kg body weight), 3) exposure to the electromagnetic field and treatment with melatonin, 4) sham exposure (with daily sham injection).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: FM
Exposure duration: continuous for 2 h/day for 45 days
Exposure 1
Main characteristics
Frequency 2.45 GHz
Type
Charakteristic
Exposure duration continuous for 2 h/day for 45 days
Modulation
Modulation type FM
Modulation frequency 50 Hz
Exposure setup
Exposure source
Chamber plexiglas cage ventilated with holes of 1 cm diameter
Setup a cage with six animals was kept in an anechoic chamber lined with radar absorbing material (attenuation 40 db); all six animals were facing the horn antenna
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.14 W/kg - estimated whole body -
power density 0.21 mW/cm² - measured - -
power 1,080 W - - - input power
power 700 W - - - output power
Reference articles
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The relative testis weight, testosterone level, the enzyme activity of lactate dehydrogenase and the sperm count were significantly decreased in EMF-exposed rats (group 1) compared to the sham exposure group and histopathological damage was found as well. A simultaneous administration of melatonin (Group 3) negated these exposure-induced effects.
All parameters of oxidative stress (levels of reactive oxygen species, carbonyl proteins, malondialdehyde and enzyme activity of xanthine oxidase) as well as the DNA damage and apoptosis were significantly increased in EMF-exposed animals (Group 1) compared to the sham exposure group. Again, simultaneous administration of melatonin (group 3) significantly reduced these parameters in comparison to group 1.
Food and water intake did not shown any differences between the groups.
The authors conclude that exposure of rats to a 2.45 GHz electromagnetic field might damage the testes via oxidative stress and that melatonin could prevent these effects.
Study character:

Study funded by

  • Council of Scientific and Industrial Research (CSIR), New Delhi, India

Related articles

  • Chauhan P et al. (2017): Microwave radiation (2.45 GHz)-induced oxidative stress: Whole-body exposure effect on histopathology of Wistar rats.
  • Djordjevic B et al. (2015): The effect of melatonin on the liver of rats exposed to microwave radiation.
  • Odaci E et al. (2015): Exposure to a 900 MHz electromagnetic field for 1 hour a day over 30 days does change the histopathology and biochemistry of the rat testis.
  • Sokolovic D et al. (2015): The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation.
  • Saygin M et al. (2015): Impact of L-carnitine and Selenium Treatment on Testicular Apoptosis in Rats Exposed to 2.45 GHz Microwave Energy.
  • Tas M et al. (2014): Long-term effects of 900 MHz radiofrequency radiation emitted from mobile phone on testicular tissue and epididymal semen quality.
  • Kumar S et al. (2014): Effect of electromagnetic irradiation produced by 3G mobile phone on male rat reproductive system in a simulated scenario.
  • Kumar S et al. (2013): Influence of electromagnetic fields on reproductive system of male rats.
  • Hanci H et al. (2013): The effect of prenatal exposure to 900-megahertz electromagnetic field on the 21-old-day rat testicle.
  • Poulletier de Gannes F et al. (2013): Rat fertility and embryo fetal development: influence of exposure to the Wi-Fi signal.
  • Jelodar G et al. (2013): The prophylactic effect of vitamin C on induced oxidative stress in rat testis following exposure to 900 MHz radio frequency wave generated by a BTS antenna model.
  • Kumar S et al. (2012): Impact of microwave at X-band in the aetiology of male infertility.
  • Al-Damegh MA (2012): Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E.
  • Kesari KK et al. (2012): Evidence for mobile phone radiation exposure effects on reproductive pattern of male rats: role of ROS.
  • Lee HJ et al. (2012): The effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on rat testicular function.
  • Kumar S et al. (2011): Influence of microwave exposure on fertility of male rats.
  • Kesari KK et al. (2011): Effects of radiofrequency electromagnetic wave exposure from cellular phones on the reproductive pattern in male wistar rats.
  • Lee HJ et al. (2010): The lack of histological changes of CDMA cellular phone-based radio frequency on rat testis.
  • Kesari KK et al. (2010): Mobile phone usage and male infertility in Wistar rats.
  • Otitoloju AA et al. (2010): Preliminary study on the induction of sperm head abnormalities in mice, Mus musculus, exposed to radiofrequency radiations from global system for mobile communication base stations.
  • Mailankot M et al. (2009): Radio frequency electromagnetic radiation (RF-EMR) from GSM (0.9/1.8GHz) mobile phones induces oxidative stress and reduces sperm motility in rats.
  • Dasdag S et al. (2008): Mobile phone exposure does not induce apoptosis on spermatogenesis in rats.
  • Yan JG et al. (2007): Effects of cellular phone emissions on sperm motility in rats.
  • Ribeiro EP et al. (2007): Effects of subchronic exposure to radio frequency from a conventional cellular telephone on testicular function in adult rats.
  • Ozguner M et al. (2005): Biological and morphological effects on the reproductive organ of rats after exposure to electromagnetic field.
  • Dasdag S et al. (2003): Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes.
  • Akdag Z et al. (1999): Effect of chronic low-intensity microwave radiation on sperm count, sperm morphology, and testicular and epididymal tissues of rats.
  • Dasdag S et al. (1999): Whole-body microwave exposure emitted by cellular phones and testicular function of rats.