研究のタイプ: 医学/生物学の研究 (experimental study)

Effect of extremely low-frequency (50 Hz) field on proliferation rate of human adipose-derived mesenchymal stem cells. med./bio.

掲載誌: J Radiobiol 2014; 1 (2): 31-37

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研究目的(著者による)

The effects of exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field on cell proliferation should be investigated.

詳細情報

The cells were divided into the following groups: 1) exposure to a 0.5 mT magnetic field for 20 minutes/day, 2) exposure to a 0.5 mT magnetic field for 40 minutes/day, 3) exposure to a 1 mT magnetic field for 20 minutes/day, 4) exposure to a 1 mT magnetic field for 40 minutes/day, 5) sham exposure for 20 minutes/day, 6) sham exposure for 40 minutes/day.
All experiments were repeated three times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 20 to 40 minutes/day for 7 days
ばく露2: 50 Hz
ばく露時間: 20 to 40 minutes/day for 7 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 20 to 40 minutes/day for 7 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 96-well plates
ばく露装置の詳細 solenoid was made of 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field at the center of the solenoid, where the cell cultures were located, was ±1%; the temperature in solenoid during exposure was controlled by a digital thermometer and magnetic field-induced heating was negligible
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 20 to 40 minutes/day for 7 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the trypan blue dye exclusion test, cell viability and cell proliferation were significantly increased in all exposure groups (groups 1-4) compared to sham exposure groups (groups 5 and 6). In the MTT test, cell viability and cell proliferation were significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the sham exposure groups. No differences in cell morphology were found between the exposure and sham exposure groups.
The authors conclude that exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field might promote cell proliferation.

研究の種別:

研究助成

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