この研究は、ゴールデンレインツリーの種子の発芽への磁界ばく露（100 G、4.3秒）の効果を調べた。発芽させる条件には、不浸透性の種皮に酸で傷をつける「酸乱切り」、休眠を破る寒冷刺激を与える「層化」がある。これらを考慮して、4つの条件で種子を処理した：1）酸乱切りとそれに続く84日間の低温層化（対照群）、2）酸乱切り（A）、21、42、63、または84日間の低温層化（B）、または磁界ばく露（C）のいずれかの単独処理、3）AまたはB処理の前または後に磁界処理、 4）A、B、Cのすべての可能な組み合わせ。各処理は、テスト毎に、20粒の種子を用いた実験を5回繰り返した。その結果、酸乱切り処理の後に磁界処理を実施した場合、磁界処理は部分的に低温層化の代用になった；酸乱切り処理後かつ84日間の低温層化の前の磁界処理は、発芽を56から97％増加させた、と報告している。
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To study the effects of an electromagnetic field on seed dormancy and germination of golden-rain tree.
Golden-rain tree seed has an impermeable testa which can be overcome by acid scarification and requires cold stratification to overcome embryo dormancy. The following seed treatments were performed: 1) control treatment of acid scarification followed by 84 days of cold stratification; 2) either acid scarification (A), cold stratification for 21, 42, 63, or 84 days (B) or electromagnetic field exposure (C) alone; 3) electromagnetic field treatment applied either before or after an A or B treatment; 4) all possible combinations of A, B, and C. Each treatment was replicated five times with 20 seeds per test.
ばく露時間: continuous for 4.3 s
seeds were treated in four groups: i) control = treatment of acid scarification followed by 84 days of cold stratification ii) either acid scarification (A) or cold stratification (C) or electromagnetic treatment (M) iii) M applied either before or after A or C treatment iv) all possible combinations of A, C and M
|ばく露時間||continuous for 4.3 s|
Germination of more than 10% occurred only when scarification was followed by cold stratification as expected. Seed germination following scarification was directly proportional to time in cold stratification. Magnetic field treatment applied after both acid scarification and cold stratification did not significantly improve germination as compared to the control (AB). When the electromagnetic field was applied between scarification and 21, 42, 63, or 84 days of cold stratification, germination increased although the effect was significant only at the last two periods. After 84 days of cold stratification, germination was 97% with the electromagnetic treatment as compared to 56% obtained with the control (AB). Seed exposure to the electromagnetic field prior to the conventional AB sequence inhibited germination.
The author concludes that an electromagnetic field treatment of 100 Gauss will partially substitute for cold stratification and significantly increase seed germination of golden-rain tree. These findings suggest that seed exposure to an electromagnetic field increases germination through an unknown effect on the cold-requiring reaction.