この研究は、海馬組織を用いて、グルタミン酸の放出と摂取に対するパルス磁界（PMF）の影響を調べた。放出は、海馬スライスを用いて検査したが、摂取については、シナプトソームでの取り込みプロセスの特性を調べた。（3）H-d-アスパラギン酸を、グルタミン作動性伝達のマーカーに用いた。その結果、海馬組織にてんかん性放電を誘発するPMF（9-15 mT）を与えると、グルタミン酸の放出および摂取がそれぞれ増幅および減衰されることが観察された；一方、神経興奮性の増加を誘発するがその時に発作を引き起こすことないことが知られている刺激パターンのPMFを与えた場合には、両方のプロセスの増幅が観察された；これらの知見は以前の報告を確認するものであり、グルタミン作動性シナプスが磁界作用の標的であることを示す、と報告している。
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To study the influence of pulsed magnetic fields on the release (during exposure) and uptake (after exposure) of glutamate in the hippocampal tissue.
The release was examined by using hippocampal slices (of mice), the uptake process was characterized by the use of synaptosomes obtained from hippocampal slices.
Patterns of magnetic fields were applied, which induced seizures in hippocampal tissue in vitro and epileptic episodes in preclinical trials.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
25 mHz
ばく露時間:
640 s
Paradigm 1
|
|
|
ばく露2:
25–100 mHz
ばく露時間:
3340 s
Paradigm 1+2
|
|
|
ばく露3:
25–250 mHz
ばく露時間:
6095 s
Paradigm 1+2+3
|
| 周波数 | 25 mHz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | 640 s |
| Additional information | Paradigm 1 |
| Additional information | Paradigm 1(0.025 Hz) is given as: 9 mT, 12 mT and 15 mT MF with 20 s on/off cycle applied 4, 4 and 8 times, respectively. The exposure time pattern of E1 is calculated as follows: 20 s pulse on + 20 s pulse off multiplied by the number of repetitions which was 4 + 4 + 8. |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | The tissue chamber was surrounded by four magnetic coils. |
| 周波数 | 25–100 mHz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | 3340 s |
| Additional information | Paradigm 1+2 |
| Additional information | Paradigm 1 (0.025 Hz) + Paradigm 2 (0.1 Hz) were applied. Paradigm 2 is given: 15 mT and another 15 mT MF with 5 s on/off cycle applied 9 and 6 times, respectively. The exposure time pattern of E2 is calculated as follows: Exposure time pattern of E1 + (5 s on + 5 s off + 20 s pause + 5 s on + 5 s off + 220 s multiplied by # of repetitions which was 9 + 6). |
| ばく露の発生源／構造 |
|
|---|
| 周波数 | 25–250 mHz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | 6095 s |
| Additional information | Paradigm 1+2+3 |
| Additional information | Paradigm 1 (0.025 Hz) + Paradigm 2 (0.1 Hz) + Paradigm 3(0.25 Hz) were applied. Paradigm 3 is given as: 15 mT with 2 s on/off cycle applied 12, 4, 3, 5 and 6 times. The exposure time pattern of E3 is calculated as follows: Exposure time pattern of E2 + (2 s on + 2 s off + 30 s pause + 2 s on + 2 s off + 10 s pause + 2 s on + 2 s off + 25 s pause + 2 s on + 2 s off + 10 pause + 2 s on + 2 s off multiplied by # of repetitions which was 12+4+3+5+6). |
| ばく露の発生源／構造 |
|
|---|
The pulsed magnetic fields (9-15 mT) amplified and attenuated the release and uptake of glutamate, respectively. However, the magnetic fields which induced an increase in neuronal excitability without concomitant seizures amplified both processes.
These data indicate that the glutamergic synapses are the target of magnetic fields action. The results show that modulation of glutamate turnover by magnetic fields correlates with overexcitability of hippocampal neurons and with epileptic episodes observed in the hippocampus of the patients exposed to magnetic fields. Pulsed magnetic field stimulation has the potential to be used as a diagnostic procedure which could help to localize the seizure-generating part of the brain.
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