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To determine the effects of low-intensity electromagnetic fields at 925 MHz on the OmpF channel-forming protein (outer membrane protein F, a transmembrane protein of E. coli).
Ion channel activity responses were monitored under different conditions: 1) in the presence of continuous electromagnetic field exposure, 2) recovering from electromagnetic field exposure (recorded after electromagnetic field was switched off), and 3) recovering from simultaneous application of electric field and electromagnetic field. The results were based on the analysis of about 25 h of recordings at 0-180 mV in 20 mV increments. The thermal effect of electromagnetic fields on channel activity was studied at temperatures of 22, 24, and 26°C, taking channel activity at 20°C as control.
4.5 cm long 1.5 cm x 1.5 cm glass chamber placed in a Faraday cage perpendicular to the E-field; two 1 mm thick aluminum antennae positioned parallel at a distance of 5.5 cm with the glass chaber between them
ion channel activity of the OmpF protein (frequency of channel gating and voltage sensitivity, channel conductance; patch-clamp technique)
porin channel in an artifical lipid bilayer system
The data showed an increase in the frequency of channel gating and voltage sensitivity under electromagnetic field exposure. These effects lasted for several milliseconds after the electromagnetic field source was terminated. However, the conductance levels of channels did not change significantly. The changes in the channel activity could be due to a minor rise in localized temperature induced by the electromagnetic field microscopically. The authors conclude that electromagnetic field exposure affects both the dynamics and conformation of the ion channel.