研究のタイプ: 医学/生物学の研究 (experimental study)

[ナノポアOmpFタンパク質のチャネル活性に対する電磁界(EMF)の影響] med./bio.

Electromagnetic field (EMF) effects on channel activity of nanopore OmpF protein.

掲載誌: Eur Biophys J 2009; 38 (8): 1069-1078

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研究目的(著者による)

To determine the effects of low-intensity electromagnetic fields at 925 MHz on the OmpF channel-forming protein (outer membrane protein F, a transmembrane protein of E. coli).

詳細情報

Ion channel activity responses were monitored under different conditions: 1) in the presence of continuous electromagnetic field exposure, 2) recovering from electromagnetic field exposure (recorded after electromagnetic field was switched off), and 3) recovering from simultaneous application of electric field and electromagnetic field. The results were based on the analysis of about 25 h of recordings at 0-180 mV in 20 mV increments.
The thermal effect of electromagnetic fields on channel activity was studied at temperatures of 22, 24, and 26°C, taking channel activity at 20°C as control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 925 MHz
Modulation type: CW
ばく露時間: not given in the article
ばく露2:
  • unspecified
Modulation type: single pulse
  • U = 200 mV
ばく露3: 925 MHz
Modulation type: single pulse, CW
ばく露時間: not given in the article

ばく露1

主たる特性
周波数 925 MHz
タイプ
  • electromagnetic field
ばく露時間 not given in the article
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • trapezoid antenna
ばく露装置の詳細 4.5 cm long 1.5 cm x 1.5 cm glass chamber placed in a Faraday cage perpendicular to the E-field; two 1 mm thick aluminum antennae positioned parallel at a distance of 5.5 cm with the glass chaber between them
パラメータ
測定量 種別 Method Mass 備考
電界強度 244 V/m maximum 計算値 - -
電界強度 4.76 V/m - 計算値 - sensed by the protein
電力密度 0.03 W/m² - 計算値 - at the membran location

ばく露2

主たる特性
周波数
  • unspecified
タイプ
  • electric field
Modulation
Modulation type single pulse
ばく露装置
ばく露の発生源/構造
  • 詳細不明
パラメータ
測定量 種別 Method Mass 備考
参照 - - - - U = 200 mV

ばく露3

主たる特性
周波数 925 MHz
タイプ
  • electromagnetic field
ばく露時間 not given in the article
Additional information plus short electric pulses
Modulation
Modulation type single pulse, CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 244 V/m maximum 計算値 - -
電界強度 4.76 V/m - 計算値 - sensed by the protein
電力密度 0.03 W/m² - 計算値 - at the membran location
参照 - - - - U = 200 mV

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The data showed an increase in the frequency of channel gating and voltage sensitivity under electromagnetic field exposure. These effects lasted for several milliseconds after the electromagnetic field source was terminated. However, the conductance levels of channels did not change significantly. The changes in the channel activity could be due to a minor rise in localized temperature induced by the electromagnetic field microscopically.
The authors conclude that electromagnetic field exposure affects both the dynamics and conformation of the ion channel.

研究の種別:

研究助成

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