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Ion channel proteins are possible sites of microwave interaction at the cell membrane level. Previous patch-clamp data indicated that low level microwave fields may alter some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes (publication 175) suggesting a possible effect of microwaves on myogenic cells.
The aim of this study was to investigate the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes, i.e. it should be investigated whether microwave effects on acetylcholine receptor determine modifications to intracellular signalling.
Experiments were performed with proliferating cells (myoblasts) or differentiated cells (myotubes). Consequences on hormonal activation of phopholipase C and phopholipase D were observed (note: acetylcholine induces phosphoinositide breakdown by acting on the acetylcholine receptor and generating inositol phosphates and diacylglycerol; phopholipase C and D can act on diacylglycerol).
|Distance between exposed object and exposure source||0.25 m|
|チャンバの詳細||Incubator maintained at 37°C and contained 5% CO2; the internal part of the incubator was a metallic box which was divided into two compartments (50 cm x 45 cm)|
|ばく露装置の詳細||Petri dishes, exposed one at a time, were placed in the upper compartment with the antenna inclined at 30° with respect to floor of the upper compartment and the E-vector was perpendicular to the incident plane|
|Additional information||The lower compartment of the incubator was shielded by metallic shelves to prevent exposure of control samples.|
Increased inositol phospholipid turnover, induced by hormonal activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, phospholipase D activation, assessed by measuring the [³H]-free choline release, was not modified by microwave exposure. The authors concluded that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6-C5 myogenic cells.