研究のタイプ: 医学/生物学の研究 (experimental study)

[筋原細胞におけるホスホリパーゼCおよびD信号伝達経路との低電力マイクロ波の相互作用] med./bio.

Low power microwave interaction with phospholipase C and D signal transduction pathways in myogenic cells.

掲載誌: Cell Biol Int 2004; 28 (10): 683-688

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研究目的(著者による)

Ion channel proteins are possible sites of microwave interaction at the cell membrane level. Previous patch-clamp data indicated that low level microwave fields may alter some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes (publication 175) suggesting a possible effect of microwaves on myogenic cells.
The aim of this study was to investigate the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes, i.e. it should be investigated whether microwave effects on acetylcholine receptor determine modifications to intracellular signalling.

詳細情報

Experiments were performed with proliferating cells (myoblasts) or differentiated cells (myotubes). Consequences on hormonal activation of phopholipase C and phopholipase D were observed (note: acetylcholine induces phosphoinositide breakdown by acting on the acetylcholine receptor and generating inositol phosphates and diacylglycerol; phopholipase C and D can act on diacylglycerol).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10.75 GHz
Modulation type: CW
ばく露時間: 20 min, 40 min or 60 min
ばく露2: 10.75 GHz
Modulation type: CW
ばく露時間: 20 min, 40 min or 60 min

General information

SAR value was estimated to be 64 mW/kg in the sample for the maximum applied power of 40 mW

ばく露1

主たる特性
周波数 10.75 GHz
ばく露時間 20 min, 40 min or 60 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 0.25 m
チャンバの詳細 Incubator maintained at 37°C and contained 5% CO2; the internal part of the incubator was a metallic box which was divided into two compartments (50 cm x 45 cm)
ばく露装置の詳細 Petri dishes, exposed one at a time, were placed in the upper compartment with the antenna inclined at 30° with respect to floor of the upper compartment and the E-vector was perpendicular to the incident plane
Additional information The lower compartment of the incubator was shielded by metallic shelves to prevent exposure of control samples.
パラメータ
測定量 種別 Method Mass 備考
電力密度 4.5 µW/cm² unspecified 計算値 - -

ばく露2

主たる特性
周波数 10.75 GHz
ばく露時間 20 min, 40 min or 60 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between exposed object and exposure source 0.25 m
パラメータ
測定量 種別 Method Mass 備考
電力密度 20 µW/cm² unspecified 計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Increased inositol phospholipid turnover, induced by hormonal activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, phospholipase D activation, assessed by measuring the [³H]-free choline release, was not modified by microwave exposure. The authors concluded that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6-C5 myogenic cells.

研究の種別:

研究助成

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