研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト線維芽細胞の核の微細構造に対するさまざまに偏波させたマイクロ波放射の影響] med./bio.

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts.

掲載誌: J Zhejiang Univ Sci B 2010; 11 (10): 801-805

この研究は、マイクロ波放射がヒト線維芽細胞の核に及ぼす影響を調べるために、3つの偏波型(直線偏波、左旋楕円偏波、右旋楕円偏波)のマイクロ波を用いたばく露実験を行なった。周波数は6.65 GHz、ばく露を受ける物体の表面での電力密度は1、10、30、100 μW / cm2、ばく露時間は10秒とした。カバースライド上に単層で増殖中のヒト線維芽細胞マイクロ波ばく露を与えた。細胞を覆う培養液の深さは約1mmであった。ばく露終了直後の細胞をオルセイン2 %(w/v)含有の45 %(w/v)酢酸溶液で染色した。実験は室温(25℃)で行われ、対照群は同じ条件で処理された。600倍の倍率でヘテロクロマチン顆粒量(HGQ)を測定した。その結果、1 μW / cm2ばく露群では影響が見られず、10および100 μW / cm2ばく露群ではHGQ量の増加が引き起こされた;ばく露レベルの上昇に伴い、より多くのクロマチン凝縮が誘発された;右旋楕円偏波の方が左旋楕円偏波よりも多くの生物活性を示した、と報告している。

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研究目的(著者による)

To study the influence of microwave irradiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization (linear, left-handed and right-handed elliptically polarized) were examined.

詳細情報

The mean heterochromatin granule quantity was calculated for 30 nuclei and each experiment was repeated three times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 36.65 GHz
ばく露時間: continuous for 10 s
linear polarized field
  • 電力密度: 1 µW/cm² (at the surface of the exposed object)
  • 電力密度: 10 µW/cm² (at the surface of the exposed object)
  • 電力密度: 30 µW/cm² (at the surface of the exposed object)
  • 電力密度: 100 µW/cm² (at the surface of the exposed object)
ばく露2: 36.65 GHz
ばく露時間: continuous for 10 s
left-handed and right-handed elliptically polarized field
  • 電力密度: 1 µW/cm² (at the surface of the exposed object)
  • 電力密度: 10 µW/cm² (at the surface of the exposed object)
  • 電力密度: 30 µW/cm² (at the surface of the exposed object)
  • 電力密度: 100 µW/cm² (at the surface of the exposed object)

ばく露1

主たる特性
周波数 36.65 GHz
タイプ
  • electromagnetic field
偏波
  • linear
ばく露時間 continuous for 10 s
Additional information linear polarized field
ばく露装置
ばく露の発生源/構造
  • self-developed semi-conductor device
ばく露装置の詳細 human fibroblasts growing in a monolayer on a cover slide, covered by 1 mm of culture medium during exposure
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 1 µW/cm² - - - at the surface of the exposed object
電力密度 10 µW/cm² - - - at the surface of the exposed object
電力密度 30 µW/cm² - - - at the surface of the exposed object
電力密度 100 µW/cm² - - - at the surface of the exposed object

ばく露2

主たる特性
周波数 36.65 GHz
タイプ
  • electromagnetic field
偏波
  • elliptical
ばく露時間 continuous for 10 s
Additional information left-handed and right-handed elliptically polarized field
ばく露装置
ばく露の発生源/構造
  • self-developed semi-conductor device + grating polarizer
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 1 µW/cm² - - - at the surface of the exposed object
電力密度 10 µW/cm² - - - at the surface of the exposed object
電力密度 30 µW/cm² - - - at the surface of the exposed object
電力密度 100 µW/cm² - - - at the surface of the exposed object

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Microwave irradiation at the intensity of 1 µW/cm² produced no effect, but exposure at the intensities of 10 and 100 µW/cm² induced an increase in heterochromatin granule quantity. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized irradiation revealed more biological activity than the left-handed polarized one.
As the left-handed elliptically polarized microwaves induced less heterochromatinization, they should be preferred for the use in various systems of radio communications.

研究の種別:

研究助成

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