研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁パルス放射によりラット睾丸でのMnSODの発現は抑制される] med./bio.

MnSOD expression inhibited by electromagnetic pulse radiation in the rat testis.

掲載誌: Electromagn Biol Med 2011; 30 (4): 205-218

本研究では、電界強度ピークピーク値が100kV/mで、反復率が50Hz(毎秒50回)の電磁パルスに雄のSprague-Dawlyラットばく露し、ラット生殖系に生じた形態学的なダメージと機能障害を定量化した。この結果、各種の影響が認められ、その影響はマンガンスーパーオキシドジスムターゼ(MnSOD)の発現抑制と関連付けられるかも知れないとの示唆を得たと結論している。

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研究目的(著者による)

To observe the effects of electromagnetic pulse exposure on the reproductive system in male rats.

詳細情報

Electromagnetic pulse is a special kind of electromagnetic irradiation that is produced as short high voltage pulses and used extensively in the medical applications and security screening and military applications. The unusual properties of electromagnetic pulses have raised concerns about potential bioeffects and health hazards to human. Therefore, this study examined the possibly damaging factors of electromagnetic pulses on the morphology of testis and sperms, endocrine changes and the metabolism in male rats.
Rats were divided into three groups: 1.) sham exposure, 2.) exposure with 3*105 pulses and 3.) exposure with 4*105 pulses. Every group consisted at of least eight rats. After 24, 48, 72 h or 1 week, rats were sacrificed and blood samples, sperm samples and testis were collected for analyses. The experiments were repeated thrice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 0 Hz–1 GHz
Modulation type: pulsed
ばく露時間: 3 x 105 or 4 x 105 pulses
  • SAR: 30 mW/kg average over mass (whole body)
  • 電界強度: 100 kV/m (at 225 cm from the source)

General information

Animals were divided into the following four groups: i) sham exposure ii) exposure to EMF (3*105 pulses) iii) exposure to EMF (4*105 pulses)

ばく露1

主たる特性
周波数 0 Hz–1 GHz
タイプ
  • electromagnetic field
波形
  • pulsed
ばく露時間 3 x 105 or 4 x 105 pulses
Modulation
Modulation type pulsed
Repetition frequency 50 Hz
Additional information

pulse width: 2.5 - 2.8 ns

ばく露装置
ばく露の発生源/構造
  • GTEMセル
  • spark gap pulse generator
Distance between exposed object and exposure source 225 cm
ばく露装置の詳細 500 cm long conical GTEM cell; rat placed in a 20 cm long polyethylene exposure holder with a diameter of 6.4 cm; holder positioned in the GTEM cell parallel with the magnetic vector at 225 cm from the EMF source
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 30 mW/kg average over mass 計算値 whole body -
電界強度 100 kV/m - - - at 225 cm from the source

Reference articles

  • Lu ST et al. (1999): Ultrawide-band electromagnetic pulses induced hypotension in rats. Dublette mit anderer PubMed-ID.

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

Acrosome reaction was significantly decreased after 24 and 48 h of exposure and abnormalities in the ultrastructure (chromatin accumulation, dilation and vacuolation of the endoplasmic reticulum) occured especially after 24 h exposure. The sperm number and sperm motility, as well as deformity and micronucleus rate did not significantly differ between the exposed and the control group.
The level of malondialdehyde increased 6 h after exposure, while the activity of the superoxide dismutase decreased after 24 h compared to the control group. Accordingly, the gene expression of the manganese superoxide dismutase was significantly decreased in the exposed group compared to the control group after 24 h.
Serum levels of testosterone and luteinizing hormone fluctuated over time in the exposure groups, while the levels in the control groups remained constant. Serum level of follicle stimulating hormone did not change in neither of the groups.
The authors summarize that morphological damage in rat's testis, as well as endocrine and metabolic dysfunctions in rats could be induced through electromagnetic pulses.

研究の種別:

研究助成

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