研究のタイプ: 医学/生物学の研究 (experimental study)

[900MHz電磁界放射への30日間ばく露後のラット精巣のプロテオーム解析] med./bio.

Analysis of rat testicular proteome following 30-day exposure to 900 MHz electromagnetic field radiation.

掲載誌: Electrophoresis 2014; 35 (23): 3331-3338

この研究は、900MHz電磁界EMF)に1日1、2、4時間、30日連続でばく露した成獣SD雄ラットから全摘した精巣の標本のプロテオームを解析した。その結果、擬似ばく露群、またはばく露群でのみ見られる合計13個のタンパク質をMALDI-TOF/TOF-MSで同定した;これらには、熱ショックタンパク質スーパーオキシドジスムターゼ、ペルオキシレドキシン-1などが合った、と報告している。

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研究目的(著者による)

The effects of a whole body exposure of rats to a 900 MHz electromagnetic field on the protein expression pattern in the testes should be investigated.

詳細情報

20 rats were divided into the following groups (n=5 each group): 1) sham exposure, 2) 1 h exposure, 3) 2 h exposure and 4) 4 h exposure. Experiments were conducted at least in triplicates.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: repeated daily exposure for 1 h on 30 consecutive days
ばく露2: 900 MHz
ばく露時間: repeated daily exposure for 2 h on 30 consecutive days
ばく露3: 900 MHz
ばく露時間: repeated daily exposure for 4 h on 30 consecutive days

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure for 1 h on 30 consecutive days
ばく露装置
チャンバの詳細 5 freely moving rats were exposed in a pure transparent polycarbonate cage of 42 cm Œ 26.5 cm Œ 15 cm near the antenna
ばく露装置の詳細 temperature was 22 ± 2°C and relative humidity was 50%
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 86 mW/cm² mean - - -
電力密度 22.8 mW/cm² minimum - - -
電力密度 146.8 mW/cm² maximum - - -
SAR 0.19 W/g mean 推定値 whole body minimum value
SAR 1.22 W/g mean 推定値 whole body maximum value

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure for 2 h on 30 consecutive days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 86 mW/cm² mean - - -
電力密度 22.8 mW/cm² minimum - - -
電力密度 146.8 mW/cm² maximum - - -
SAR 0.19 W/g mean 推定値 whole body minimum value
SAR 1.22 W/g mean 推定値 whole body maximum value

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure for 4 h on 30 consecutive days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 86 mW/cm² mean - - -
電力密度 22.8 mW/cm² minimum - - -
電力密度 146.8 mW/cm² maximum - - -
SAR 0.19 W/g mean 推定値 whole body minimum value
SAR 1.22 W/g mean 推定値 whole body maximum value

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences were found in weight and total protein concentration of testes between the groups. Two-dimensional gel electrophoresis revealed 16 protein spots that were found only in the sham-exposed group, while 18 protein spots existed only in the exposed groups. No clear differences in the protein expression pattern were found between the exposure groups. 6 proteins which were found only in the sham-exposed group and 7 proteins which were found only in the exposed groups were identified, e.g. several heat shock proteins in the exposed groups and a superoxide dismutase in the sham exposure.
The authors conclude that an exposure of rats to a 900 MHz electromagnetic field could induce the expression or the absence of proteins related to stress and stress protection, respectively, in the testes. Further studies are needed to confirm and extend the results of this preliminary study.

研究の種別:

研究助成

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