研究のタイプ: 医学/生物学の研究 (experimental study)

[がん細胞の増殖は特定の変調周波数によって抑制される] med./bio.

Cancer cell proliferation is inhibited by specific modulation frequencies.

掲載誌: Br J Cancer 2012; 106 (2): 307-313

がん患者に対して極めて低く安全なレベルの振幅変調電磁界を、口内用スプーン状プローブを介して放射すると、治療効果が誘導されることがあるとする臨床的証拠が、著者らの先行研究(本データベース収録済み)で得られている。本研究では、この新規アプローチのメカニズムを理解するため、in vivo条件下で再現できるようにデザインされたin vitroばく露系を用いて、以前特定された腫瘍に特異的な変調周波数を有する27.12MHzの高周波電磁界がん細胞ばく露させた。この結果、肝細胞がん細胞および乳がん細胞の増殖は、それぞれに特異的な変調周波数により有意に抑制されたが、この周波数は、非悪性肝細胞および乳房上皮細胞の増殖には影響を及ぼさなかった。著者らは、これらの知見は、特定の変調周波数を用いて、正常な組織に影響を及ぼすことなく、がん細胞増殖を制御する新規メカニズムを明らかにするものであり、がん治療に対し広い影響をもたらすものであると考えられる、としている。

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研究目的(acc. to editor)

To study the effects of amplitude modulated electromagnetic fields on the cell proliferation of cancer cells. The authors aimed to investigate the mechanism of action of amplitude modulated electromagnetic fields used in previous clinical studies for the treatment of cancer patients (Barbault et la. 2009 and Costa et la. 2011).

詳細情報

There is clinical evidence that very low levels of amplitude modulated electromagnetic fields administered via an intrabuccal spoon-shaped probe may elicit therapeutic responses in patients with cancer. To understand the mechanism of this approach, hepatocellular carcinoma cells were exposed to 27.12 MHz radiofrequency electromagnetic fields to replicate the in vivo conditions. Additionally, cancer cells were exposed to tumour-specific modulation frequencies, previously identified by "biofeedback" methods in patients with a diagnosis of cancer (Barbault et la. 2009). Using such a patient-based "biofeedback" approach, Barbault et la. 2009 identified strikingly similar frequencies among patients with the same type of cancer and observed that patients with a different type of cancer had "biofeedback" responses to different frequencies.
Control modulation frequencies consisted of randomly chosen modulation frequencies.
Two different in vitro exposure systems were used to expose cells in culture (parallel plate capacitor and TEM cell).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 27.12 MHz
Modulation type: AM
ばく露時間: continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
hepatocellular carcinoma-specific modulation frequencies
  • SAR: 0.03 W/kg (average at the monolayer)
  • SAR: 0.05 W/kg (for additional experiments)
  • SAR: 0.1 W/kg (for additional experiments)
  • SAR: 1 W/kg (for additional experiments)
ばく露2: 27.12 MHz
Modulation type: AM
ばく露時間: continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
randomly chosen frequencies
  • SAR: 0.03 W/kg (average at the monolayer)
  • SAR: 0.05 W/kg (for additional experiments)
  • SAR: 0.1 W/kg (for additional experiments)
  • SAR: 1 W/kg (for additional experiments)
ばく露3: 27.12 MHz
Modulation type: AM
ばく露時間: continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
breast tumor-specific modulation frequencies
  • SAR: 0.03 W/kg (average at the monolayer)
  • SAR: 0.05 W/kg (for additional experiments)
  • SAR: 0.1 W/kg (for additional experiments)
  • SAR: 1 W/kg (for additional experiments)
ばく露4: 27.12 MHz
Modulation type: AM
ばく露時間: continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
  • SAR: 0.4 W/kg

ばく露1

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
Additional information hepatocellular carcinoma-specific modulation frequencies
Modulation
Modulation type AM
Modulation frequency 410.23 Hz–20.365284 kHz
ばく露装置
ばく露の発生源/構造
  • parallel plate capacitor
ばく露装置の詳細 four 14 cm x 21 cm parallel plate capacitors each consisting of 3 brass plates connected via a coaxial cable to a signal generator and placed inside an incubator
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.03 W/kg - - - average at the monolayer
SAR 0.05 W/kg - - - for additional experiments
SAR 0.1 W/kg - - - for additional experiments
SAR 1 W/kg - - - for additional experiments

ばく露2

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
Additional information randomly chosen frequencies
Modulation
Modulation type AM
Modulation frequency 504.28 Hz–22.401812 kHz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.03 W/kg - - - average at the monolayer
SAR 0.05 W/kg - - - for additional experiments
SAR 0.1 W/kg - - - for additional experiments
SAR 1 W/kg - - - for additional experiments

ばく露3

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
Additional information breast tumor-specific modulation frequencies
Modulation
Modulation type AM
Modulation frequency 181.821 Hz–19.406211 kHz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.03 W/kg - - - average at the monolayer
SAR 0.05 W/kg - - - for additional experiments
SAR 0.1 W/kg - - - for additional experiments
SAR 1 W/kg - - - for additional experiments

ばく露4

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 h/day, 3 h/day or 6 h/day for 7 days or 3 h/day for 3 days
Additional information it is not specified clearly in the article whether the modulation frequencies from Exposure 1, 2 and 3 were used here also or not
Modulation
Modulation type AM
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two TEM cells placed inside an incubator; 2 x 6 tissue culture dishes (à 35 mm) per TEM cell
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.4 W/kg - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The growth of hepatocellular carcinoma and breast cancer cells was significantly decreased by hepatocellular carcinoma- and breast cancer-specific modulation frequencies, respectively. However, the same frequencies did not affect cell proliferation of non-malignant hepatocytes or normal breast epithelial cells. Inhibition of hepatocellular carcinoma cell proliferation was associated with downregulation of XCL2 and PLP2. Furthermore, hepatocellular carcinoma-specific modulation frequencies disrupted the mitotic spindle.
These data uncovered a novel mechanism controlling the growth of cancer cells at specific modulation frequencies without affecting normal tissues, which may have broad implications in oncology.

研究の種別:

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