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研究目的(acc. to editor)
To study the effects of amplitude modulated electromagnetic fields on the cell proliferation of cancer cells. The authors aimed to investigate the mechanism of action of amplitude modulated electromagnetic fields used in previous clinical studies for the treatment of cancer patients (Barbault et la. 2009 and Costa et la. 2011).
詳細情報
There is clinical evidence that very low levels of amplitude modulated electromagnetic fields administered via an intrabuccal spoon-shaped probe may elicit therapeutic responses in patients with cancer. To understand the mechanism of this approach, hepatocellular carcinoma cells were exposed to 27.12 MHz radiofrequency electromagnetic fields to replicate the in vivo conditions. Additionally, cancer cells were exposed to tumour-specific modulation frequencies, previously identified by "biofeedback" methods in patients with a diagnosis of cancer (Barbault et la. 2009). Using such a patient-based "biofeedback" approach, Barbault et la. 2009 identified strikingly similar frequencies among patients with the same type of cancer and observed that patients with a different type of cancer had "biofeedback" responses to different frequencies. Control modulation frequencies consisted of randomly chosen modulation frequencies. Two different in vitro exposure systems were used to expose cells in culture (parallel plate capacitor and TEM cell).
four 14 cm x 21 cm parallel plate capacitors each consisting of 3 brass plates connected via a coaxial cable to a signal generator and placed inside an incubator
分子生合成: general gene expression (RNA-seq); validation of PLP2 and XCL2 gene expression (Real-time PCR)
細胞生存力／細胞分裂/増殖: growth inhibition (via ³H-thymidine incorporation or determination of cell proliferation/cell viability (based on ATP quantitation; luminescent commercial assay))
The growth of hepatocellular carcinoma and breast cancer cells was significantly decreased by hepatocellular carcinoma- and breast cancer-specific modulation frequencies, respectively. However, the same frequencies did not affect cell proliferation of non-malignant hepatocytes or normal breast epithelial cells. Inhibition of hepatocellular carcinoma cell proliferation was associated with downregulation of XCL2 and PLP2. Furthermore, hepatocellular carcinoma-specific modulation frequencies disrupted the mitotic spindle. These data uncovered a novel mechanism controlling the growth of cancer cells at specific modulation frequencies without affecting normal tissues, which may have broad implications in oncology.
