研究のタイプ: 医学/生物学の研究 (experimental study)

[神経膠芽腫細胞(U87)に対する超低周波パルス電磁界(ELF-PEMF)の影響] med./bio.

Effects of extremely low-frequency pulsed electromagnetic fields (ELF-PEMFs) on glioblastoma cells (U87).

掲載誌: Electromagn Biol Med 2017; 36 (3): 238-247

この研究は、多形性膠芽腫(GBM)の細胞株(U87)のイン・ビトロでの細胞周期アポトーシスおよび生存率に対する超低周波パルス電磁界(ELF-PEMF)の影響を周波数、振幅およびばく露時間を変えて調べた。実験は、3通りのばく露時間(2、4、24時間)×5通りのばく露条件(I(無ばく露対照)、II(50Hz、100G)、III(100Hz、100G)、IV(10Hz、50G)、V(50Hz、50G))の15通りとした。ばく露終了後に、形態学的特性、細胞生存率細胞周期およびアポトーシス関連タンパク質遺伝子発現(Cyclin-D1、P53およびCaspase-3)を調べた。その結果、24時間ばく露では、II群で生存率およびCyclin-D1発現の増加が見られた一方、IIIおよびIV群ではそれらは減少した;またIII群ではp53およびCaspase-3発現が増加した;V群では何も変化はなかった、などの所見を報告している。

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研究目的(著者による)

The effects of exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field on cell cycle, apoptosis and cell viability should be investigated in view of a possible use in cancer therapy.

詳細情報

Cells were divided into the following groups: exposure to a magnetic field with 1) 50 Hz/10 mT, 2) 100 Hz/10 mT, 3) 10 Hz/5 mT, 4) 50 Hz/5 mT and 5) control group. Each group was investigated after 2, 4 and 24 hours of exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: for 2, 4 and 24 hours
ばく露2: 100 Hz
ばく露時間: for 2, 4 and 24 hours
ばく露3: 10 Hz
ばく露時間: for 2, 4 and 24 hours
ばく露4: 50 Hz
ばく露時間: for 2, 4 and 24 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 2, 4 and 24 hours
Additional information pulsed and/or continuous square waves (?)
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 polyvinyl chloride (PVC) cell culture flasks or plates with 96 wells were placed between two identical coils in a vertical field: each coil was boxed in a plexiglass chamber with dimensions of 160 x 160 x 50 mm3 by maintaining a coaxial distance of 40 mm; cooling water system around coils avoided temperature rise; cells were always placed on the same shelves of the cell culture incubator
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - 測定値 - ± 1.5 mT

ばく露2

主たる特性
周波数 100 Hz
タイプ
  • magnetic field
ばく露時間 for 2, 4 and 24 hours
Additional information pulsed and/or continuous square waves (?)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - 測定値 - ± 1.5 mT

ばく露3

主たる特性
周波数 10 Hz
タイプ
  • magnetic field
ばく露時間 for 2, 4 and 24 hours
Additional information pulsed and/or continuous square waves (?)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT - 測定値 - ± 1 mT

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 2, 4 and 24 hours
Additional information pulsed and/or continuous square waves (?)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT - 測定値 - ± 1 mT

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The most distinct effects were found after 24 hours of exposure. Here, the cell viability and Cyclin-D1 protein expression were significantly increased in group 1 compared to the control group, while they were significantly decreased in groups 2 and 3. Protein expression of P53 and Caspase-3 was significantly increased in group 2 after 24 hours compared to the control group.
Cell morphology showed significant alterations in groups 1-3 after 24 hours compared to the control group with decreased mass, increased cell surface and changed shape.
The authors conclude that exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field may alter cell cycle, apoptosis and cell viability depending on exposure duration, field frequency and intensity.

研究の種別:

研究助成

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