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The effects of exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field on cell cycle, apoptosis and cell viability should be investigated in view of a possible use in cancer therapy.
Cells were divided into the following groups: exposure to a magnetic field with 1) 50 Hz/10 mT, 2) 100 Hz/10 mT, 3) 10 Hz/5 mT, 4) 50 Hz/5 mT and 5) control group. Each group was investigated after 2, 4 and 24 hours of exposure.
|ばく露装置の詳細||polyvinyl chloride (PVC) cell culture flasks or plates with 96 wells were placed between two identical coils in a vertical field: each coil was boxed in a plexiglass chamber with dimensions of 160 x 160 x 50 mm3 by maintaining a coaxial distance of 40 mm; cooling water system around coils avoided temperature rise; cells were always placed on the same shelves of the cell culture incubator|
The most distinct effects were found after 24 hours of exposure. Here, the cell viability and Cyclin-D1 protein expression were significantly increased in group 1 compared to the control group, while they were significantly decreased in groups 2 and 3. Protein expression of P53 and Caspase-3 was significantly increased in group 2 after 24 hours compared to the control group.
Cell morphology showed significant alterations in groups 1-3 after 24 hours compared to the control group with decreased mass, increased cell surface and changed shape.
The authors conclude that exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field may alter cell cycle, apoptosis and cell viability depending on exposure duration, field frequency and intensity.