研究のタイプ: 医学/生物学の研究 (experimental study)

[高周波界による赤血球溶血] med./bio.

Erythrocyte hemolysis by radiofrequency fields.

掲載誌: Bioelectromagnetics 1985; 6 (3): 313-322

この研究は、ヘパリン処理したウサギ血液赤血球を用いて、溶血作用に対する高周波電磁界ばく露の影響をインビトロ実験した。その結果、赤血球は、4V / cmを上回る電界強度の、50または100MHzのRF電磁界への2時間ばく露により溶血した;電界強度9V / cmの10MHzのRFばく露で、同等の大きさの作用がもたらされた;試料温度は22.5℃±0.2℃に維持され、熱または温度勾配の見かけ上の関与はなかった;溶血作用のメカニズムは不明である、と報告している。

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研究目的(著者による)

To investigate the effects of continuous wave radiofrequency irradiation of 10, 50, and 100 MHz on cation permeability and hemolysis of rabbit erythrocytes in vitro.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10 MHz
Modulation type: CW
ばく露時間: continuous for 2 h
ばく露2: 50 MHz
Modulation type: CW
ばく露時間: continuous for 2 h
ばく露3: 100 MHz
Modulation type: CW
ばく露時間: continuous for 2 h

ばく露1

主たる特性
周波数 10 MHz
タイプ
  • electromagnetic field
特性
  • guided field
偏波
  • circular
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • 円筒導波管
  • coaxial cell exposure chamber
チャンバの詳細 The coaxial cell exposure chamber was a mechanically modified version of the system described in [Guy, 1977]. It consisted of an annular region having a volume of 5 ml with stainless steel walls (3.34 and 3.84 cm radius) and a Teflon bottom. Samples could be exposed to relatively uniform (15% variation) electric field strengths at temperatures controlled using a high-rate circulating-liquid heat exchanger which allowed duplicating the temperature history of RF- and sham-exposed samples.
Sham exposure A sham exposure was conducted.
Additional information Two samples were RF and sham exposed in identical exposure chambers at 22.5 ± 0.2 °C. A third sample was sham exposed at 20 ± 0.1 °C in an incubator, primarily as a reference for K+ release [Cleary et al., 1982]. A fourth control sample in a capped standard 10-ml plastic culture tube was placed together with the third one. All exposures were performed simultaneously. The interior surfaces of the RF chambers and culture tubes were siliconised prior to each experiment.
パラメータ
測定量 種別 Method Mass 備考
電界強度 6.3 V/cm - 測定値 - -
電界強度 9 V/cm - 測定値 - -

ばく露2

主たる特性
周波数 50 MHz
タイプ
  • electromagnetic field
特性
  • guided field
偏波
  • circular
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 1 V/cm - 測定値 - -
電界強度 4.6 V/cm - 測定値 - -
電界強度 9 V/cm - 測定値 - -

ばく露3

主たる特性
周波数 100 MHz
タイプ
  • electromagnetic field
特性
  • guided field
偏波
  • circular
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 1 V/cm - 測定値 - -
電界強度 4.5 V/cm - 測定値 - -
電界強度 9 V/cm - 測定値 - -

Reference articles

  • Cleary SF et al. (1982): [ウサギ赤血球のX-バンドマイクロ波へのばく露影響]
  • Guy AW (1977): [温度および電磁界強度の制御下における電磁界への細胞培養のばく露方法]

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Potassium and sodium release were not altered by exposure. Hemoglobin release as an indicator for hemolysis was statistically significant increased at 50 and 100 MHz at a field strengh of 9 V/cm. The mechanism of the observed hemolytic effect is unknown.

研究の種別:

研究助成

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