この研究は、ヘパリン処理したウサギ血液の赤血球を用いて、溶血作用に対する高周波電磁界ばく露の影響をインビトロ実験した。その結果、赤血球は、4V / cmを上回る電界強度の、50または100MHzのRF電磁界への2時間ばく露により溶血した；電界強度9V / cmの10MHzのRFばく露で、同等の大きさの作用がもたらされた；試料温度は22.5℃±0.2℃に維持され、熱または温度勾配の見かけ上の関与はなかった；溶血作用のメカニズムは不明である、と報告している。
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To investigate the effects of continuous wave radiofrequency irradiation of 10, 50, and 100 MHz on cation permeability and hemolysis of rabbit erythrocytes in vitro.
|ばく露時間||continuous for 2 h|
|チャンバの詳細||The coaxial cell exposure chamber was a mechanically modified version of the system described in [Guy, 1977]. It consisted of an annular region having a volume of 5 ml with stainless steel walls (3.34 and 3.84 cm radius) and a Teflon bottom. Samples could be exposed to relatively uniform (15% variation) electric field strengths at temperatures controlled using a high-rate circulating-liquid heat exchanger which allowed duplicating the temperature history of RF- and sham-exposed samples.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Two samples were RF and sham exposed in identical exposure chambers at 22.5 ± 0.2 °C. A third sample was sham exposed at 20 ± 0.1 °C in an incubator, primarily as a reference for K+ release [Cleary et al., 1982]. A fourth control sample in a capped standard 10-ml plastic culture tube was placed together with the third one. All exposures were performed simultaneously. The interior surfaces of the RF chambers and culture tubes were siliconised prior to each experiment.|
Potassium and sodium release were not altered by exposure. Hemoglobin release as an indicator for hemolysis was statistically significant increased at 50 and 100 MHz at a field strengh of 9 V/cm. The mechanism of the observed hemolytic effect is unknown.