研究のタイプ: 医学/生物学の研究 (experimental study)

[ルシフェラーゼ溶液に対する940 MHz電磁界の影響:構造、機能、および誘電性の研究] med./bio.

Effects of 940 MHz EMF on Luciferase solution: structure, function, and dielectric studies.

掲載誌: Bioelectromagnetics 2013; 34 (6): 489-498

この研究は、方形導波管ばく露システムを設計し、ルシフェラーゼ(ホタル科のLampyris turkestanicusから抽出した発光酵素)の構造と活性に対する940MHz携帯電話電磁界の影響を調べた。方形導波管は長さ600mm、開口部の幅と高さは170mmであり、940MHzのTE01モード進行波を1Wで入力した時、管内部のほぼ一様な垂直方向電界レベルは約6 V/ m(水平方向はその1/9の大きさである)であり、バイアルに入れた試料のSARはおよそ0.02W/ kgであった。結果として、無ばく露に比べばく露したルシフェラーゼの活性は有意に高かった;動的光散乱法で計測した流体力学的半径は小さくなった(20 nm vs. 47 nm±5%);λ=360 nmでの混濁度の計測により会合体形成は低下する傾向が示された;ばく露の前と後での0.2 から20 GHzの範囲の誘電率計測における誘電定数の変化は、電磁界による脱会合力に関連していた、と報告している。

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研究目的(著者による)

To study the effects of mobile phone frequency (940 MHz) electromagnetic fields on luciferase structure and activity (as a model enzyme).

詳細情報

Luciferase activities of exposed and control samples with different pre-exposure incubation times on ice (10 and 50 min) were compared.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 940 MHz
ばく露時間: exposure duration not given clearly (30 min?)
  • 電力: 1 W (input power)
  • 電界強度: 6 V/m effective value
  • SAR: 0.021 W/g average over mass (1 g) (along the length of the sample tube)

ばく露1

主たる特性
周波数 940 MHz
タイプ
  • electromagnetic field
ばく露時間 exposure duration not given clearly (30 min?)
ばく露装置
ばく露の発生源/構造
チャンバの詳細 5 cm plastic vial (0,5 mm wall thickness) placed in the X-shaped sample holder
ばく露装置の詳細 waveguide unit (aluminium box 600 mm in length and 170 mm in width and height); two probes generated the electromagnetic wave of 940 MHz at one end of the waveguide, and the other end was matched with the same configuration (only the vertical probe was used); four aluminum stubs were used for impedance matching of waveguide and elimination of standing wave pattern; X-shape Teflon sample holder for five samples (horizontally oriented; only central position was used) placed in the centre of the waveguide; waveguide was placed in an incubator (25°C); at each end of the waveguide, four pores were covered by metal meshes and equipped with fans to circulate the air inside the waveguide;
Additional information control samples were shielded with aluminium cylinders (1 cm in thickness) and placed in the incubator outside of the waveguide at the same temperature
パラメータ
測定量 種別 Method Mass 備考
電力 1 W - - - input power
電界強度 6 V/m effective value 計算値 - -
SAR 0.021 W/g average over mass 計算値 1 g along the length of the sample tube

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

The luciferase activity of exposed samples was significantly higher than that of unexposed samples. The particle size (mean diameter) of the control samples was significantly larger than that of the exposed samples. The exposed samples showed a less tendency to form aggregates. The change in the dielectric properties of the exposed luciferase solution was related to the disaggregation potency of the applied field.
The authors conclude that the data suggest that direct interactions with luciferase molecules and its dipole moment were responsible for the reduced aggregation and enhanced luciferase activity following exposure to the electromagnetic field.

研究の種別:

研究助成

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