研究のタイプ: 医学/生物学の研究 (experimental study)

[ルシフェラーゼ産生HEK細胞の安定株の生物発光と酸化反応に対する940 MHz EMFの影響] med./bio.

Effects of 940 MHz EMF on bioluminescence and oxidative response of stable luciferase producing HEK cells.

掲載誌: Photochem Photobiol Sci 2014; 13 (7): 1082-1092

この研究は、ホタルルシフェラーゼ遺伝子を内在的に有する安定な細胞株(HEK293T)に対する携帯電話周波数の電磁界RF-EMF、940MHz)の影響を調べた。出力1Wの導波管ばく露システムで、ペトリ皿平均SAR 0.09 W kg-1のばく露を与えた。ばく露時間は15、30、45、60、90分間とした。その結果、30、45分間群では、内因性ルシフェラーゼ活性が低下し、60分間群では細胞溶解物のルシフェラーゼ活性は無ばく露群より高くなった、などを報告している。

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研究目的(著者による)

The effects of an acute exposure of transgenic human embryonic kidney cells to a 940 MHz electromagnetic field on the luciferase enzyme activity and oxidative stress should be investigated.

詳細情報

The firefly luciferase gene was transfected into human embryonic kidney cells as the enzyme activity of luciferase is a sensitive marker for cellular stress.
Cells were either sham exposed or exposed for up to 90 minutes. All results represent means of 3 repeated experiments with 3 measurements, respectively.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 940 MHz
ばく露時間: continuous for up to 90 minutes

ばく露1

主たる特性
周波数 940 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for up to 90 minutes
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell monolayer inside a 35 mm petri dish
ばく露装置の詳細 waveguide unit consisted of an aluminum box (60 x 17 x 17 cm) with one probe at each end generating the field; 4 discs (stubs) were used for impedance matching; reactangular waveguide was placed in an incubator and equipped with two fans working in opposite directions at both ends for temperature control (37 ± 0.3°C in incubator; temperature change in dishes was 0.07±0.03 °C (range 0.02-0.1°C) during exposure); field inhomogeneity was below 30%
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.09 W/kg mean 計算値 - -
電界強度 10 V/m effective value - - across petri dish
電力 1 W - - - input power

Reference articles

  • Sefidbakht Y et al. (2013): [ルシフェラーゼ溶液に対する940 MHz電磁界の影響:構造、機能、および誘電性の研究]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The luciferase enzyme activity was significantly decreased after 30 and 45 minutes and significantly increased after 60 minutes of exposure compared to sham exposure.
The reactive oxygen species content was significantly increased after 15, 30 and 45 minutes of exposure compared to sham exposure. The enzyme activity of superoxide dismutase was significantly increased after 30 and 45 minutes of exposure compared to sham exposure. The enzyme activity of catalase was significantly increased after 15, 30, 45 and 60 minutes of exposure compared to sham exposure. The content of reduced glutathione was significantly increased after 45 minutes of exposure, whereas the content of malondialdehyde was significantly reduced after 45 minutes compared to sham exposure.
The enzyme activity of caspase 3 and 7 was significantly increased after 45 and 90 minutes, but not after 60 minutes of exposure compared to sham exposure, showing no distinct indication for apoptosis.
The authors conclude, that an acute exposure of transgenic human embryonic kidney cells to a 940 MHz electromagnetic field could cause oxidative stress which is followed by an anti-oxidative response, what is represented by the luciferase enzyme activity.

研究の種別:

研究助成

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