この研究は、ヒツジの脳の大脳皮質組織から調製されたシナプトソーム（神経前終末部）懸濁液を実験材料に用いて、シナプスのアセチルコリン（ACh）濃度に対する超低周波電磁界（ELF-EMF）ばく露の影響を調べた。ACh濃度は、アセチルコリンエステラーゼ（AChE）の酵素活性アッセイにより測定した。多種多様なELF-EMFばく露を行った。周波数は50 Hz - 230 Hz、磁束密度は0.1 mT - 1.7mT、ばく露時間は15 - 120分間とした。得られたデータは、ニューラルネットワークベースのソフトウェアであるInform 4.02によって分析され、9つの3D曲線によりAChE活性パターンを予測した。結果として、これらの曲線は、磁束密度が1.2mTから1.7mTの範囲、周波数が50Hzから90Hzの範囲の場合、AChE活性が低下することを示した、と報告している。
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To examine the effects of extremely low frequency magnetic fields on synaptic acetylcholine concentrations through an acetylcholinesterase enzyme assay.
The enzyme acetylcholinesterase controls the concentration of acetylcholine in the synaptic clefts by hydrolizing acetylcholin to choline and acetate. Cholinergic synapses are involved in important brain functions such as learning, memory and cognition. An acetylcholinesterase dysfunction could lead to cognitive disorders.
The effect of different frequencies, exposure times and magnetic field intensities on the enzyme activity of acetylcholinesterase was tested. Data were analyzed by a software to predict acetylcholinesterase acticity pattern.
|ばく露時間||up to 120 minutes (15, 30, 45, 60, 90, 120 minutes)|
|ばく露装置の詳細||tube of synaptosomal suspension was placed in the area of the two parallel coils with 35 cm distance and 35 cm internal diameter made of 1000 turns of coated 1.3 mm copper wire (magnetic field lines ran vertically), the device was supplied by a power generator, and frequency and intensity of magnetic field were monitored by a sensor connected to a digital multimeter and oscilloscope, temperature fluctuations were negliable|
|Sham exposure||A sham exposure was conducted.|
The enzyme activity of the acetylcholinesterase changed according to the intensity and the frequency of the magnetic field. In the intensity range from 1.2 mT to 1.7 mT and frequency range from 50 Hz to 90 Hz, the enzyme activity was significantly decreased compared to the unexposed control. However, in the intensity range from 0.3 mT to 0.6 mT and frequency range from 50 Hz to 90 Hz, as well as in the intensity range from 0.8 mT to 1.2 mT and frequency range from 150 Hz to 230 Hz, the enzyme activity was significantly increased in comparison to the control. The exposure duration had no significant effect.
The authors conclude that exposure to extremely low frequency magnetic fields could be a promising tool in the treatment of cholinergic disorders.