研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁界存在下でのシナプトソームのアセチルコリンエステラーゼ活性変動パターン] med./bio.

Synaptosomal acetylcholinesterase activity variation pattern in the presence of electromagnetic fields.

掲載誌: Int J Biol Macromol 2014; 65: 8-15

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研究目的(著者による)

To examine the effects of extremely low frequency magnetic fields on synaptic acetylcholine concentrations through an acetylcholinesterase enzyme assay.

詳細情報

The enzyme acetylcholinesterase controls the concentration of acetylcholine in the synaptic clefts by hydrolizing acetylcholin to choline and acetate. Cholinergic synapses are involved in important brain functions such as learning, memory and cognition. An acetylcholinesterase dysfunction could lead to cognitive disorders.
The effect of different frequencies, exposure times and magnetic field intensities on the enzyme activity of acetylcholinesterase was tested. Data were analyzed by a software to predict acetylcholinesterase acticity pattern.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50–230 Hz
ばく露時間: up to 120 minutes (15, 30, 45, 60, 90, 120 minutes)

ばく露1

主たる特性
周波数 50–230 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 up to 120 minutes (15, 30, 45, 60, 90, 120 minutes)
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 tube of synaptosomal suspension was placed in the area of the two parallel coils with 35 cm distance and 35 cm internal diameter made of 1000 turns of coated 1.3 mm copper wire (magnetic field lines ran vertically), the device was supplied by a power generator, and frequency and intensity of magnetic field were monitored by a sensor connected to a digital multimeter and oscilloscope, temperature fluctuations were negliable
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.3 mT minimum 測定値 - -
磁束密度 0.5 mT - 測定値 - -
磁束密度 0.7 mT - 測定値 - -
磁束密度 0.9 mT - 測定値 - -
磁束密度 1.1 mT - 測定値 - -
磁束密度 1.3 mT - 測定値 - -
磁束密度 1.5 mT - 測定値 - -
磁束密度 1.7 mT maximum 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The enzyme activity of the acetylcholinesterase changed according to the intensity and the frequency of the magnetic field. In the intensity range from 1.2 mT to 1.7 mT and frequency range from 50 Hz to 90 Hz, the enzyme activity was significantly decreased compared to the unexposed control. However, in the intensity range from 0.3 mT to 0.6 mT and frequency range from 50 Hz to 90 Hz, as well as in the intensity range from 0.8 mT to 1.2 mT and frequency range from 150 Hz to 230 Hz, the enzyme activity was significantly increased in comparison to the control. The exposure duration had no significant effect.
The authors conclude that exposure to extremely low frequency magnetic fields could be a promising tool in the treatment of cholinergic disorders.

研究の種別:

研究助成

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