研究のタイプ: 医学/生物学の研究 (experimental study)

[細胞増殖および遺伝子発現に対する超低周波磁界の影響] med./bio.

Effect of extremely low frequency magnetic fields on cell proliferation and gene expression.

掲載誌: Bioelectromagnetics 2015; 36 (7): 506-516

この研究は、種々の細胞株(MCF10A、MCF7、Jurkat、NIH3T3)を用いて、その細胞増殖細胞死、生存力、DNA合成速度に対する超低周波磁界(ELF-MF:60Hz、1mT:ばく露時間4または16時間)の影響を調べた。次に遺伝子発現に対する影響をELF-MF(60Hz、2mT:ばく露時間16時間)で調べた。その結果、細胞数、生存力、DNA合成速度について、JurkatおよびNIH3T3細胞では一定の変化は見られなかったが、 MCF10A およびMCF7細胞では有意な低下が見られた;細胞死に対するELF-MFの影響はどの細胞株でも見られなかった;遺伝子発現については、遺伝子PMAIP1の発現に変化が確認された、と報告している。

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研究目的(著者による)

The effects of exposure of 4 different cell lines to a 60 Hz magnetic field on cell growth and gene expression should be investigated.

詳細情報

MCF10 A cells were investigated 1, 2, 3 and 4 days after exposure, the remaining cell lines were investigated only 3 days after exposure.
A separate sham exposure group was used for each exposure condition. A positive control (gamma radiation, 4 and 6 Gy) was used for gene expression analysis. All experiments were repeated thrice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 4 hours
MCF10 A only
ばく露2: 60 Hz
ばく露時間: continuous for 4 hours
MCF10 A only
ばく露3: 60 Hz
ばく露時間: continuous for 4 hours or 16 hours
all cell lines
ばく露4: 60 Hz
ばく露時間: continuous for 16 hours
MCF7 only

General information

for the positive control group, cells were exposed to 4 and 6 Gy of gamma radiation from a 137 Cs gamma ray source at a dose rate of 3.81 Gy/min

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information MCF10 A only
ばく露装置
ばく露の発生源/構造
  • 詳細不明
チャンバの詳細 60 mm culture dish
ばく露装置の詳細 one dish per condition was placed in an incubator equipped with the exposure system; after exposure, the cell dish was transferred into an incubator without exposure system until investigation; temperature was maintained constantly at 37 ± 0.3°C, humidity was maintained at ~95%, CO2 was 5%
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.1 mT - - - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information MCF10 A only
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - - - -

ばく露3

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours or 16 hours
Additional information all cell lines
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露4

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 16 hours
Additional information MCF7 only
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to 0.1 mT and 0.5 mT magnetic fields did not show any effects.
When exposed to 1 mT, MCF10 A and MCF7 cells showed consistent and significant decreases in cell numbers, cell viability and cell proliferation compared to sham exposed cells, whereas Jurkat cells and NIH3T3 cells did not show consistent effects. No significant effects on cell death were found in any cell line.
In MCF7 cells exposed to 2 mT, cell numbers were significantly decreased and the cell cycle distribution was significantly altered compared to sham exposed cells . Apoptosis was not affected by the exposure. In gene expression analysis, PMAIP1-expression was significantly upregulated compared to sham exposed cells.
The authors conclude that exposure to a 60 Hz magnetic field might affect cell growth in some cell lines, possibly in association with a cell cycle delay and an increased expression of PMAIP1.

研究の種別:

研究助成

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