研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界(ELF-MF)により仲介される血管新生の阻害] med./bio.

Inhibition of angiogenesis mediated by extremely low-frequency magnetic fields (ELF-MFs).

掲載誌: PLoS One 2013; 8 (11): e79309

この研究は、著者の仮説である「ELF-EMFによる抗血管新生作用」のメカニズムを調査するために、内皮細胞モデルに50 Hz、2 mTの正弦波磁界MFばく露を与え、その細胞の状態、増殖運動性、細管形成能力を測定した。内皮細胞モデルには、ヒト臍帯静脈血管内皮細胞HUVECおよびマウス膵臓由来内皮細胞株MS-1を用いた。その結果、マウスに移植されたMS-1細胞は急速な腫瘍増殖を明らかに示すが、MFばく露を与えたMS-1細胞移植されたマウスにおいてはそのような増殖は有意に低下した;MFばく露MS-1細胞移植されたマウス由来の腫瘍組織学的分析では、血管腫のサイズ、血液充満空間、および出血の減少が示された;MFばく露MS-1細胞のインビトロでの増殖も有意に抑制された;また、MFばく露は、HUVECにおける増殖、遊走、および細管様構造形成のプロセスを低下させることも示された;また、MFばく露は、血管内皮増殖因子(VEGF2)の発現と活性化レベルを有意に低下させることが示され、細胞膜に配置されたVEGF受容体に対するMFの直接的または間接的な影響が示唆された、と報告している。

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研究目的(著者による)

To evaluate the angiogenesis in two endothelial cell models exposed to an extremely low frequency magnetic field in vivo and in vitro.

詳細情報

The inhibition of angiogenesis could represent a possible therapeutic strategy in diseases where an excessive angiogenesis is involved (for example cancer).
For the in vivo experiments, exposed (72 hours) or not exposed mouse endothelial cells (MS-1, tumor promoting) cells were injected subcutaneously into the flanks of mice and tumor development was observed for 7 days. For the in vitro experiments, MS-1 cells and human umbilical endothelial cells (HUVECs) cells were exposed for up to 24 and 72 hours, respectively.
Vascular endothelial growth factor (VEGF) is a signal protein that (amongst others) stimulates angiogenesis.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for up to 24 h
HUVECs
ばく露2: 50 Hz
ばく露時間: continuous for 72 hours
MS-1 cells

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for up to 24 h
Additional information HUVECs
ばく露装置
ばく露の発生源/構造
チャンバの詳細 Helmholtz coils were placed in an incubator at 37°C, 5 % CO2, 95 % air and 100 % relative humidity
ばく露装置の詳細 Helmholtz coils had a mean radius of 13 cm, in each coil the number of turns was 800 with a 2 mm² wire giving a resulting resistance of 2.4 Ohm and an inductance of ca. 39 mH, mean vertical distance between the coils was 13.5 cm; simultaneous exposure of a maximum of 6 culture plates
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT minimum 測定値 - -
磁束密度 2 mT maximum 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 72 hours
Additional information MS-1 cells
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

Reference articles

  • Kirschvink JL (1992): [均一磁界とダブル巻きコイルシステム:生体電磁気実験のための設計改善技術]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Mice inoculated with magnetic field exposed MS-1 cells developed significant smaller tumors than mice inoculated with sham exposed MS-1 cells. The histopathological examination of the tumors indicated a reduction of hemangioma (a benign tumor) size, of blood-filled spaces, and in hemorrhage in mice with exposed cells compared to mice with sham exposed MS-1 cells. Also in vitro analysis of MS-1 cells showed that the magnetic field exposure significantly decreased cell proliferation.
In exposed human umbilical vein endothelial cells, cell proliferation, migration and formation of tubule-like formation was significantly dereased compared to sham exposed cells. Additionally, the protein expression level of vascular endothelial growth factor and phosphorylated vascular endothelial growth factor was significantly down-regulated in exposed human umbilical vein endothelial cells compared to sham exposed ones.
The authors conclude that the magnetic field exposure reduced the ability of endothelial cells to form new vessels in vivo and in vitro, probably via an vascular endothelial growth factor pathway. These findings could help to develop therapeutic applications for the treatment of diseases with involved angiogenesis.

研究の種別:

研究助成

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