研究のタイプ: 医学/生物学の研究 (experimental study)

[クワガタ(昆虫、甲虫目)の触角葉のニューロンの生体電気活性に対する2つの異なる波形のELF磁界の影響] med./bio.

Effects of two different waveforms of ELF MFs on bioelectrical activity of antennal lobe neurons of Morimus funereus (Insecta, Coleoptera).

掲載誌: Int J Radiat Biol 2015; 91 (5): 435-442

この研究は、超低周波磁界(ELF MF:50Hz、2mT)にばく露したクワガタの成虫の触角葉のニューロンの生体電気活性を記録し、分析した。ELF MFの波形正弦波および矩形波ばく露時間は5、10、15分間とした。その結果、正弦波では何も影響が見られなかった;矩形波では、10、15分間ばく露で、ニューロン活動の変化が見られた、と報告している。

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研究目的(著者による)

The effects of exposure of longhorn beetles to a sine wave or square wave 50 Hz magnetic field on the neuronal activity in the antennal lobe (part of the insect brain which receives the input from the olfactory sensory neurons on the antenna) should be investigated.

詳細情報

The longhorn beetle was used as it had already been known to be sensitive to static magnetic fields (see Todorovic et al. 2007) and moreover, because it is particularly relevant as an endangered species in Europe. 22 beetles were divided into the following two groups: 1) exposure to sine waves (n=12), 2) exposure to square waves (n=10). Each beetle was subsequently exposed for 5, 10 and 15 minutes at 40 minutes intervals. The neuronal activity was recorded for 5 minutes directly before and after an exposure session, respectively. The very first record was defined as the sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 5, 10 and 15 minutes with 40 minutes intervals in between within 2 h
sine waves
ばく露2: 50 Hz
ばく露時間: continuous for 5, 10 and 15 minutes with 40 minutes intervals in between within 2 h
square waves

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 5, 10 and 15 minutes with 40 minutes intervals in between within 2 h
Additional information sine waves
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 1 cm
ばく露装置の詳細 animals were attached to a plexiglas horizontal plate with an opening for the head in a Faraday cage with 23°C and humidity of 50-60%; a cylindrical Teflon coil (l=70 mm, R=30 mm) containing enameled copper wire coiled around a soft iron core with 8 mm in diameter was placed at a distance of about 1 cm from the insect's left antenna
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT mean 測定値 - on the surface of the head
磁束密度 19 mT - 測定値 - on top of the coil iron core

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 continuous for 5, 10 and 15 minutes with 40 minutes intervals in between within 2 h
Additional information square waves
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT mean 測定値 - on the surface of the head
磁束密度 19 mT - 測定値 - on top of the coil iron core

Reference articles

  • Ilijin L et al. (2011): [磁界の急性影響に対するマイマイガの幼虫の背内側A1’および背外側L2’神経分泌ニューロンへの応答]
  • Pozzi D et al. (2007): [神経芽細胞の形態に対する50 Hz磁界ばく露の影響]
  • Todorovic D et al. (2007): [Morimus funereus(Coleoptera, Cerambycidae)に神経集団の活動変化に対する磁界効果の検出方法]
  • Prolic Z et al. (2003): [低周波磁界にばく露されたMorimus funereus (甲虫類,カミキリムシ科)の行動の変異]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • insect brain
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences were found in the neuronal activity of the antennal lobe between the exposure to a sine wave magnetic field and the sham exposure. For the exposure to the square wave magnetic field, significant but reversible changes were detected after the exposure for 15 minutes compared to the measurement after the exposure for 10 minutes.
The results indicate that exposure of longhorn beetles to a square wave 50 Hz magnetic field could influence the neuronal activity in the antennal lobe.

研究の種別:

研究助成

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