この研究は、７週齢のBALB/c×CBA/Ca F1ハイブリッドの雄マウスに超低周波磁界（50Hz；10、100、300μT）のばく露を2時間または15時間与えた。一分子PCR法を用いて、ばく露群および擬似ばく露群のマウスの精子および血液標本から抽出したDNAでの突然変異出現頻度を測定した。突然変異出現頻度は、X線（1Gy）の急性ばく露によるものと比較した。その結果、血液のDNAでの突然変異出現頻度は、ばく露群と擬似ばく露群で有意差がなかった；精子での頻度は、ばく露群でかろうじて有意な増加が見られたが、量反応関係はなかった；X線では、精子および血液のどちらでも有意な増加が示された、と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
The effects of exposure of mice to a 50 Hz magnetic field on mutation induction in sperms and blood should be investigated.
Mice were divided into 8 groups (n=5 each): 1) sham exposure, 2) exposure to 1 Gy of X-rays (positive control), exposure to a magnetic field with 3) 10 µT for 2 hours, 4) 10 µT for 15 hours, 5) 100 µT for 2 hours, 6) 100 µT for 15 hours, 7) 300 µT for 2 hours and 8) 300 µT for 15 hours. Testes and blood samples were collected from all animals 12 weeks after exposure.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 2 h
|
|
|
ばく露2:
50 Hz
ばく露時間:
continuous for 15 h
|
|
|
ばく露3:
50 Hz
ばく露時間:
continuous for 2 h
|
|
|
ばく露4:
50 Hz
ばく露時間:
continuous for 15 h
|
|
|
ばく露5:
50 Hz
ばく露時間:
continuous for 2 h
|
|
|
ばく露6:
50 Hz
ばく露時間:
continuous for 15 h
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 2 h |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | two pairs of Helmholtz coils generated a homogenous magnetic field (± 5% within the centre of the coils occupied by the mice); the horizontal pair (overall diameter of 54 cm) and vertical pair (overall diameter of 99 cm) each consisted of 150 turns of 1 mm copper wire wound on a frame made of Tufnol resin; earthed screen was wound around all coils to prevent stray electric fields |
| Sham exposure | A sham exposure was conducted. |
| Additional information | X-ray radiation (1 Gy) in positive control was delivered at a rate of 0.5 Gy/min |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 10 µT | - | 測定値 | - | ± 5% |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 15 h |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 10 µT | - | 測定値 | - | ± 5% |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 2 h |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 100 µT | - | 測定値 | - | ± 5% |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 15 h |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 100 µT | - | 測定値 | - | ± 5% |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 2 h |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 300 µT | - | 測定値 | - | ± 5% |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 15 h |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 300 µT | - | 測定値 | - | ± 5% |
None of the exposure groups (3-8) showed any significant differences in the mutation frequencies in blood and sperms. Combining the data of all exposure groups (3-8), a small but significant increase in the mutation frequency in sperms could be found compared to the sham exposure. However, a dose-response relationship could not be established.
The authors conclude that exposure of mice to a 50 Hz magnetic field has only a minor effect on mutation induction in the testes at most, at least under the given experimental conditions.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
