研究のタイプ: 医学/生物学の研究 (experimental study)

[50Hz磁界には反応せず、非常に低線量のX線に反応したマウス胎仔の脳のアポトーシスおよびDNA二重鎖切断の増加] med./bio.

Increased apoptosis and DNA double-strand breaks in the embryonic mouse brain in response to very low-dose X-rays but not 50 Hz magnetic fields.

掲載誌: J R Soc Interface 2014; 11 (100): 20140783

この研究は、50Hz磁界またはX線への妊娠母マウス(C57BL/6)の全身ばく露により、胎仔の脳でDNA二重鎖切断およびアポトーシスが起きるか否かを調べた。X線急性ばく露は10、20、50、200mGy、50Hz磁界は100 µT(連続的に2時間)、300 µT(断続的に15時間)、300 µT(連続的に15時間)の3条件のばく露および疑似ばく露とした。その結果、10–200mGyのX線ばく露群の胎児の脳では、対照群に比べ、DNA二重鎖切断およびアポトーシスが有意に増加した;一方、磁界ばく露群の胎児の脳では、対照群との有意差が見られなかった、と報告している。

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研究目的(著者による)

DNA double-strand breaks and apoptosis in fetal mouse brain as possible effects of an whole body exposure to a 50 Hz magnetic field or X-rays should be investigated.

詳細情報

Up to four pregnant mice were exposed to acute doses of 10, 25, 50, 100 and 200 mGy of X-rays or under different exposure conditions to a magnetic field and sham exposure, respectively. Cage controls were conducted in parallel to each treatment. Subsequently, the animals were sacrificed, fetuses were removed and cryosections of the investigated brain areas were produced and investigated. For each exposure condition and control, two sections from each of three embryos from each of three mothers (a total of 18 sections) were analyzed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 2 hours
ばく露2: 50 Hz
ばく露時間: intermittent for 15 hours (5 min on and 10 min off)
ばく露3: 50 Hz
ばく露時間: continuous for 15 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 2 hours
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 During exposure (or sham exposure), up to four pregnant mice were housed in a non-metallic cage between a pair of Helmholtz coils.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 intermittent for 15 hours (5 min on and 10 min off)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 300 µT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 15 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 300 µT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

A statistically significant increase in formation of double-strand breaks and apoptosis was detected in samples after X-ray radiation with 10 - 200 mGy compared to the control. In contrast to that, no significant differences compared to the control were found in samples of animals exposed to the magnetic fields.
Therefore, the authors conclude that any generation of double-strand breaks and apoptosis in fetal mouse brain by an exposure to magnetic fields is lower than by radiation with 10 mGy X-rays.

研究の種別:

研究助成

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