この研究は、U937［ヒトマクロファージ様］細胞株の生存力に対する静磁界および交流磁界、またはピューロマイシン（PMC）［抗生物質の一つ］との組合せの影響の評価を実施した。交流磁界は12Hz、25Hz、35Hz、50Hzで、磁界は6.5mT（rms）に調整した。磁界および／またはPMCにばく露したサンプルの応答を、細胞の生存係数S（対照群の生存細胞に対するばく露群の生存細胞の比率で定義、フローサイトメトリで分析）で示した。PMC処理群では、生存細胞の比率は約1/3に低下した。磁界単独ばく露は、ばく露の種類にかかわらず、細胞の生存力に影響を及ぼさなかったが、静磁界または交流磁界とPMCとの同時作用は細胞の生存力に有意に影響を及ぼした。最も強い致死的な影響は、6mTの静磁界、および35Hzの交流磁界で認められた。
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The effects of (co-)exposure of U937 cells to a static magnetic field, an alternating extremely low frequency magnetic field and/or puromycin on the cell viability should be investigated.
Puromycin is an antibiotic, which unselectively inhibits protein biosynthesis in cells, finally causing cell death.
Cells were divided into the follwoing groups: 1) only exposure to a 2 mT static magnetic field, or to a 2) 6 mT static magnetic field, 3) only exposure to an alternating 12 Hz magnetic field, 4) 25 Hz magnetic field, 5) 35 Hz magnetic field, or 6) 50 Hz magnetic field, 7) only puromycin treatment (100 µg/ml), 8) sham exposure. Moreover, several combinations of a static magnetic field, an alternating magnetic field and/or puromycin were tested.
At least 6 experiments were conducted for each measurement.
| ばく露 | パラメータ |
|---|---|
ばく露1:
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
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ばく露2:
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
|
|
ばく露3:
12 Hz
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
|
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ばく露4:
25 Hz
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
|
|
ばく露5:
35 Hz
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
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|
ばく露6:
50 Hz
ばく露時間:
continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h
|
|
in case of a co-exposure to a static field and an alternating field, the alternating field was perpendicularly oriented towards the static field; the background static magnetic field was < 0.05 ± 0.01 mT and the background alternating magnetic field was < 0.03 ± 0.01 mT
| 周波数 |
|
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 2.5 h or intermittent 3 times for 2.5 h, respectively, at intervals of 24 h |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | tubes |
| ばく露装置の詳細 | coils with samples were placed in incubator; magnetic field was homogeneous in test tubes with a accuracy of a few percent; temperature inside the coils was kept at 37 ± 0.1°C |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 2 mT | - | 測定値 | - | - |
Exposure to either a static (groups 1 and 2), an alternating magnetic field (groups 3 - 6) or co-exposure to any combination of both types of magnetic fields had no influence on the cell viability in comparison to the sham exposure.
Exposure to puromycin alone decreased cell viability distinctly compared to the sham exposure. No synergistic effect was observed for a co-exposure to any combination of puromycin and a static or alternating magnetic field.
However, a triple co-exposure to a static and an alternating magnetic field together with puromycin, led to a significant decrease in cell viability compared to the puromycin exposure alone, with the most profound effect in the combination of a triple exposure of cells to a static field with 6 mT, an alternating field with 35 Hz and puromycin.
The authors conclude that co-exposure of U937 cells to a static field and an alternating extremely low frequency field might enhance the cytotoxic effects of puromycin.
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