研究のタイプ: 医学/生物学の研究 (experimental study)

[聴覚事象関連電位のミスマッチ陰性電位成分に対する超低周波電界への出生前および後のばく露の影響:酸化ストレスとの関連] med./bio.

Effects of pre- and postnatal exposure to extremely low-frequency electric fields on mismatch negativity component of the auditory event-related potentials: Relation to oxidative stress.

掲載誌: Electromagn Biol Med 2016; 35 (3): 245-259

この研究は、ラットに出生前後の期間に50Hzの電界(12kV/m、1時間/日)のばく露を与え、ばく露終了後にミスマッチ陰性電位(MMN)を測定した。ラットを4群:対照群、出生前ばく露群(Pr群)、出生後ばく露群(Po群)出生前+後ばく露群(PP群)に分け、Pr、PP群は母ラットの全妊娠期間中、PP、Po群は出生後の3ヵ月間、ばく露を受けた。MMN測定後、脳組織標本酸化ストレス指標を検査した。その結果、全てのばく露群において、対照群に比べMMN振幅が減少した;脂質酸化物である4-ヒドロキシ-2-ノネナールのレベルは、対照群に比べPo群では有意に増加したが、Pr、Po群に比べPP群では有意に低下した;カルボニル化タンパク質レベルは、対照、Pr、Po群に比べPP群で有意に低下した、と報告している。

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研究目的(著者による)

The effects of prenatal and/or postnatal exposure of rats to a 50 Hz electric field on mismatch negativity in the EEG and oxidative brain damage should be investigated.

詳細情報

Mismatch negativity was investigated as an indicator for brain function.
Pregnant rats were divided into 4 groups: 1) only prenatal exposure, 2) only postnatal exposure, 3) prenatal and postnatal exposure, 4) sham exposed control group. 22 days after birth, male young animals were separated from their mothers, and 90 days after birth, 10 male rats per group were investigated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 1 hour/day during gestation until birth (22 days)
prenatal exposure
ばく露2: 50 Hz
ばく露時間: 1 hour/day for 90 days after birth
postnatal exposure
ばく露3: 50 Hz
ばく露時間: 1 hour/day during gestation until birth (22 days) and for 90 days after birth
prenatal and postnatal exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 1 hour/day during gestation until birth (22 days)
Additional information prenatal exposure
ばく露装置
ばく露の発生源/構造
  • plate capacitor
チャンバの詳細 plastic cage (the animals' home cage; 42.5 cm Œ 26.5 cm Œ 18.5 cm)
ばく露装置の詳細 the parallel plate capacitor consisted of custom made copper plates (50 cm Œ 80 cm) plated with zinc (2 mm thickness); in order to produce an uniform field, the corners of the plates were rounded; plates were placed upright on wooden stands and positioned parallel to each other (50 cm distance); cables from AC voltage transformer were connected to the center of the plates on their outer surfaces to preserve field homogeneity; one pregnant rat or four pups in one cage were exposed at one time; the cage was placed in the center between the two plates at equal distance from the plates; the cage was suitable for free movement of pups (and mothers?) (remark EMF-Portal: the direction of the electric field is specified as "vertical" in the article although the figures show a setup with a horizontal field)
Sham exposure A sham exposure was conducted.
Additional information rats of the prenatal exposure group were sham exposed for 90 days after birth
パラメータ
測定量 種別 Method Mass 備考
電界強度 12 kV/m - 計算値 - 11.7 - 11.89 kV/m

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 1 hour/day for 90 days after birth
Additional information postnatal exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Additional information rats of the postnatal exposure group were sham exposed during gestation
パラメータ
測定量 種別 Method Mass 備考
電界強度 12 kV/m - 計算値 - 11.7 - 11.89 kV/m

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 1 hour/day during gestation until birth (22 days) and for 90 days after birth
Additional information prenatal and postnatal exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 12 kV/m - 計算値 - 11.7 - 11.89 kV/m

Reference articles

  • Aydin MA et al. (2006): [ラットのヒ骨神経挫滅治癒モデルでの神経再生に対する50 Hz電界の連続ばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The mismatch negativity amplitude was significantly decreased in all exposure groups compared to the control group, indicating an effect of the electric field exposure on the EEG of rats.
Lipid peroxidation was significantly increased in the postnatal exposure group (group 2) compared to the control group, while it was significantly decreased in the prenatal and postnatal exposure group (group 3) compared to prenatal (group 1) and postnatal exposure group.
The protein carbonyl levels were significantly decreased in group 3 compared to all other groups.
No apoptosis was found in any group and there were no differences observed in general health, food intake or body weight between the groups.
The authors conclude that prenatal and/or postnatal exposure of rats to a 50 Hz electric field might decrease mismatch negativity amplitude in the EEG. This could be partly explained by increased lipid peroxidation.

研究の種別:

研究助成

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