研究のタイプ: 医学/生物学の研究 (experimental study)

[抗酸化能、脂質過酸化、視覚誘発電位に対する様々な強度の超低周波電界の影響] med./bio.

The effect of different strengths of extremely low-frequency electric fields on antioxidant status, lipid peroxidation, and visual evoked potentials.

掲載誌: Electromagn Biol Med 2012; 31 (4): 436-448

この研究は、Wistar系雌ラット(月齢3ヵ月)に電界強度12 kV/m(E12群)または18 kV/m(E18群)を1日1時間、14日間与え、視覚誘発電位VEP)、チオバルビツール酸反応物質(TBARS:脂質過酸化反応の指標)、総抗酸化能(TAS)、総酸化能(TOS)、酸化ストレス指数(OSI)を調べた。ラット30匹を無作為に対照群、E12群、E18群に分けた。その結果、E12群、E18群の方が対照群より、脳および網膜のTBARS、TOS、OSI が有意に高かった;さらにE18群の方がE12群より、TBARS レベルが高かった;E12群、E18群の方が対照群より、脳および網膜のTASレベルが有意に低かった;ばく露群の方が対照群より全てのVEP成分の時間が有意に延びた;さらにE18群の方がE12群より、VEP成分の全ての潜時が増加した、などを報告している。

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研究目的(著者による)

To examine the effects of extremely low frequency electric fields on visual evoked potentials and oxidative stress in the retina and brain of rats.

詳細情報

Three groups were examined: 1) control group, 2.) exposure to an electric field of 12 kV/m and 3.) exposure to an electric field of 18 kV/m. After two weeks of exposure, visual evoked potentials were recorded under anesthesia. The day after, rats were sacrificed and brain samples were taken for analysis of the oxidative stress.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 1 h/day for two weeks
ばく露2: 50 Hz
ばく露時間: continuous for 1 h/day for two weeks

General information

Rats were exposed in three groups: i) 12 kV/m ii) 18 kV/m iii) control group

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 continuous for 1 h/day for two weeks
ばく露装置
ばく露の発生源/構造
  • parallel plate capacitor
チャンバの詳細 rats were allowed to move freely in a plastic cage, placed between the plates
ばく露装置の詳細 parallel copper plates (50 x 80 cm) were plated with zinc (2 mm thickness) and positioned parallel to each other, placed upright on wooden stands; cables were connected to the center of the plates on their outer surfaces to preserve homogeneity of the electric field
Sham exposure A sham exposure was conducted.
Additional information the plates were spaced at 50 cm in distance for 12 kV /m electric field
パラメータ
測定量 種別 Method Mass 備考
電界強度 12 kV/m - 測定値 - 11.700 kV/m - 11.890 kV/m

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 continuous for 1 h/day for two weeks
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Additional information the plates were spaced at 33.3 cm in distance for 18 kV /m electric field
パラメータ
測定量 種別 Method Mass 備考
電界強度 18 kV/m - 測定値 - 17.568 kV/m - 17.853 kV/m

Reference articles

  • Aydin MA et al. (2006): [ラットのヒ骨神経挫滅治癒モデルでの神経再生に対する50 Hz電界の連続ばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The lipid peroxidation, the total oxidant status and the oxidative stress index were significantly increased in the brain and retina of exposed rats when compared to the control group while the total antioxidant status was significantly decreased. Regarding lipid peroxidation a dose related effect was observed: The values of the 18 kV/m-exposed group were significantly higher than those of the 12 kV/m-exposed group.
The latencies of the visual evoked potentials were significantly elongated in the exposed groups compared to the control group. Additionally, the latencies were significantly longer in the 18 kV/m exposed group than in the 12 kV/m exposed group.
The authors suggest, that exposure to extremely low frequency electric fields could change visual evoked potentials in rats and that these changes could be explained by increased oxidative stress levels.

研究の種別:

研究助成

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