研究のタイプ: 医学／生物学の研究 (experimental study)

[CWおよびGSM変調の900MHz無線周波へのヒトリンパ球のインビトロばく露：増殖、アポトーシス、ミドコンドリア膜電位の研究] med./bio.

In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential

著者: Capri M, Scarcella E, Fumelli C, Bianchi E, Salvioli S, Mesirca P, Agostini C, Antolini A, Schiavoni A, Castellani G, Bersani F, Franceschi C
掲載誌: Radiat Res 2004; 162 (2): 211-218
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この研究は、ヒト免疫細胞の機能に対して、GSM信号などのRF電磁界が非熱的影響を与えるか否かを調べた。健常ドナー（27±5歳）の末梢血単核細胞（PBMC）に、900MHzのインビトロばく露（GSM信号：SAR 76 mW/kgまたは正弦波連続波（CW）；SAR 70mW/kg）をTEMセルにて、1日1時間、3日間行った。調べたエンドポイントは、（１）マイトジェン誘導性の増殖、（２）細胞周期の促進、（３）自発性アポトーシスおよび2-deoxy-D-ribose（dRib）誘発性アポトーシス、（４）自発性、dRib誘発性アポトーシス中のミトコンドリア膜電位変化。その結果、GSM信号ばく露群では、低レベルマイトジェン刺激した場合は細胞増殖が若干低下し、膜ホスファチジルセリン分布を変化させた場合は細胞数が若干増加した；一方、細胞周期、ミトコンドリア膜電位、アポトーシス感受性には影響が見られなかった；CW電磁界ばく露群では、全てのエンドポイントにおいて影響がなかった、と報告している。

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研究目的（著者による）

To study the nonthermal effects of radiofrequency fields on human immune cells (peripheral blood mononuclear cells) exposed to a Global System for Mobile Communication (GSM) signal generated by a commercial cellular phone and by a sinusoidal non-modulated signal (continuous wave).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 1 h/day during 48 h or 72 h of culture
  • SAR: 76 mW/kg mean (unspecified)
ばく露2: 900 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 1 h/day during 48 h or 72 h of culture
  • SAR: 70 mW/kg mean (unspecified)
ばく露3: 900 MHz
Modulation type: CW
ばく露時間: repeated daily intermittent exposure, 30 min on/30 min off/30 min on per day, see add. info
  • SAR: 70 mW/kg mean (unspecified)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 1 h/day during 48 h or 72 h of culture
Modulation
Modulation type pulsed
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The TEM cell (107 x 51 x 51 cm, septum height 23 cm) was positioned vertically inside a thermostatic room maintained at 37°C.
ばく露装置の詳細 Microplates were located in the middle section of the cell on the right and on the left side of the septum on a thin plastic shelf (2 mm).
Additional information A 96 well microplate (127 x 84 x 15 mm) or two 24 well microplates (127 x 84 x 22 mm) placed one upon the other were used. Well diameters and volume of culture medium were 6.4 or 16 mm and 200 µl or 2 ml, respectively.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 76 mW/kg mean 計算値 unspecified -
測定および計算の詳細

The TEM cell reflection and transmission coefficients were measured using a network analyser, and the transmission coefficient was used to determine the cell input power. The signal at the amplifier output was measured with a peak power meter. The field inside the microplate was computed by the FDTD technique. Microplates were irradiated by a plane wave with the E-field (1 V/m) tangential to the bottom. Peak and averaged values of the field in the cell layer were computed, and the field dispersion around the average value was calculated by statistical analysis. Most of the cells were irradiated at the averaged E-field level with a small dispersion.

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
特性
  • guided field
ばく露時間 repeated daily exposure, 1 h/day during 48 h or 72 h of culture
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
パラメータ
測定量 値 種別 Method Mass 備考
SAR 70 mW/kg mean 計算値 unspecified -

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
特性
  • guided field
ばく露時間 repeated daily intermittent exposure, 30 min on/30 min off/30 min on per day, see add. info
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
Additional information The first hour of exposure took place immediately after cell seeding and treatment with mitogenic or apoptosis inducers, the second and third hours took place after 24 or 48 h of culture.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 70 mW/kg mean 計算値 unspecified -

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Results obtained from cells exposed to a GSM-modulated radiofrequency field showed a slight decrease in cell proliferation when the cells were stimulated with the lowest mitogen concentration. Cell cycle phases, mitochondrial membrane potential and susceptibility to apoptosis were found to be unaffected by the radiofrequency field.
When cells were exposed to a continuous wave radiofrequency field, no significant modifications were revealed in comparison with sham-exposed cells for all the endpoints investigated.

研究の種別:

研究助成

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