Study type: Medical/biological study (experimental study)

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues. med./bio.

Published in: Indian J Biochem Biophys 2008; 45 (5): 326-331

Aim of study (acc. to author)

To study the effects of extremely low frequency electromagnetic fields on free radical levels, natural antioxidant systems and respiratory burst system activities in heart and liver tissues of guinea pigs.

Background/further details

62 male guinea pigs were divided into seven groups: Group I (control group), II (1 mT, 4 h/day), III (1 mT, 8 h/day), IV (2 mT, 4 h/day), V (2 mT, 8 h/day), VI (3 mT, 4 h/day), and VII (3 mT, 8 h/day).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 4 h/day or 8 h/day - for 5 days

General information

animals were treated in 7 groups: i) sham ii) 1 mT for 4 h/d iii) 1 mT for 8 h/d iv) 2 mT for 4 h/d v) 2 mT for 8 h/d vi) 3 mT for 4 h/d vii) 3 mT for 8 h/d

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 4 h/day or 8 h/day - for 5 days
Exposure setup
Exposure source
Setup vertical pairs of circular Helmholtz coils; animals kept in 26 cm x 22 cm x 10 cm polycarbonate cages positioned at the center of the coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -
magnetic flux density 2 mT - measured - -
magnetic flux density 3 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Malondialdehyde levels increased in liver in groups II and IV, but decreased in group VII for both liver and heart tissues. Nitric oxide levels declined in heart in groups II and III and in liver in groups III, V, and VI, but increased in liver in group VII. Glutathione levels increased in heart in groups II, IV, V, and VI, and in liver in groups VI and VII, but decreased in groups II and IV in liver.
Myeloperoxidase enzyme activity decreased in liver in groups III, IV, VI and VII with respect to control group and in heart tissues in groups II, III and IV; however, there was a significant increase myeloperoxidase enzyme activity in heart in group VII.
In conclusion, the data demonstrated that electromagnetic fields induced oxidative stress by enhancing lipid peroxidation and reducing glutathione level which act as a free radical scavanger only for magnetic field of 1 and 2 mT with exposure period of 4 h. Nitric oxid levels and myeloperoxidase enzyme activity decreased in all exposure groups except for 3 mT, 8 h.

Study character:

Study funded by

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