To study the effects of extremely low frequency electromagnetic fields on free radical levels, natural antioxidant systems and respiratory burst system activities in heart and liver tissues of guinea pigs.
62 male guinea pigs were divided into seven groups: Group I (control group), II (1 mT, 4 h/day), III (1 mT, 8 h/day), IV (2 mT, 4 h/day), V (2 mT, 8 h/day), VI (3 mT, 4 h/day), and VII (3 mT, 8 h/day).
animals were treated in 7 groups: i) sham ii) 1 mT for 4 h/d iii) 1 mT for 8 h/d iv) 2 mT for 4 h/d v) 2 mT for 8 h/d vi) 3 mT for 4 h/d vii) 3 mT for 8 h/d
|Exposure duration||4 h/day or 8 h/day - for 5 days|
Malondialdehyde levels increased in liver in groups II and IV, but decreased in group VII for both liver and heart tissues. Nitric oxide levels declined in heart in groups II and III and in liver in groups III, V, and VI, but increased in liver in group VII. Glutathione levels increased in heart in groups II, IV, V, and VI, and in liver in groups VI and VII, but decreased in groups II and IV in liver.
Myeloperoxidase enzyme activity decreased in liver in groups III, IV, VI and VII with respect to control group and in heart tissues in groups II, III and IV; however, there was a significant increase myeloperoxidase enzyme activity in heart in group VII.
In conclusion, the data demonstrated that electromagnetic fields induced oxidative stress by enhancing lipid peroxidation and reducing glutathione level which act as a free radical scavanger only for magnetic field of 1 and 2 mT with exposure period of 4 h. Nitric oxid levels and myeloperoxidase enzyme activity decreased in all exposure groups except for 3 mT, 8 h.