Investigation of protein expression changes were replicated in ten experiments. Results were compared with the data of an earlier study that used 900 MHz GSM mobile phone irradiation (Nylund and Leszczynski 2004).
|Exposure duration||continuous for 1 h|
|SAR||2 W/kg||average over time||-||-||-|
Eight of 900 detected proteins were differentially expressed in exposed cells. Three out of these eight proteins were identified using MALDI-MS (spermidine synthase, glucose-regulated protein (GRP78) and proteasome subunit alpha type 1 (PSA1)). Due to the lack of the availability of commercial antibodies the authors were only able to further examine expression of GRP78. Using SDS-PAGE and Western blot method they were not able to confirm the result obtained for GRP78 using two-dimensional electrophoresis. Additionally, no effect of 1800 MHz GSM exposure was found on the protein expression of vimentin and Hsp27. These proteins were affected by the 900 MHz GSM exposure in earlier studies (see "Related articles").
In conclusion, the data suggest that the 900 MHz and 1800 MHz GSM exposures might affect the expression of some proteins in the EA.hy926 cell line. The discrepancy observed here between the expression changes of GRP78 detected with different methods confirms the importance of validation of the results obtained with two-dimensional electrophoresis using other methods, e.g. Western blot.