MCF7 human breast cancer cells were exposed to 849 MHz radiofrequency irradiation at average SAR values of either 2 W/kg or 10 W/kg, and the changes in protein expression profiles were monitored via 2D-DIGE high throughput technique. Some protein spots which showed altered expression without reproducibility in triplicate were validated using non-high throughput techniques (electrospray ionization tandem mass spectrometry, RT-PCR, Western blot).
Heat shock treatment (43°C, 30 min) and exposure to gamma radiation (0.5 Gy) were used for positive controls.
| Frequency | 849 MHz |
|---|---|
| Type | |
| Exposure duration | continuous for 1 h/day on 3 consecutive days |
| Exposure source |
|
|---|---|
| Chamber | temperature controlled 420 mm x 317 mm x 100 mm rectangular cavity exposure chamber |
| Sham exposure | A sham exposure was conducted. |
The protein expression profiles of the MCF7 cells were not significantly altered following radiofrequency exposure. None of the protein spots on the 2D-DIGE gels showed reproducible changes in three independent experiments. Additionally, there was no alteration in the mRNA and protein levels of three protein spots showing altered expression without reproducibility.
The authors conclude that it seems unlikely that radiofrequency exposure modulates the protein expression profile as they were unable to observe any significant and reproducible changes in the protein expression profiles of the exposed MCF7 cells using high throughput and non-high throughput techniques,
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