Study type: Medical/biological study (experimental study)

Two-dimensional electrophoretic analysis of radio-frequency radiation-exposed MCF7 breast cancer cells. med./bio.

Published in: J Radiat Res 2010; 51 (2): 205-213

Aim of study (acc. to author)

MCF7 human breast cancer cells were exposed to 849 MHz radiofrequency irradiation at average SAR values of either 2 W/kg or 10 W/kg, and the changes in protein expression profiles were monitored via 2D-DIGE high throughput technique. Some protein spots which showed altered expression without reproducibility in triplicate were validated using non-high throughput techniques (electrospray ionization tandem mass spectrometry, RT-PCR, Western blot).

Background/further details

Heat shock treatment (43°C, 30 min) and exposure to gamma radiation (0.5 Gy) were used for positive controls.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 849 MHz
Exposure duration: continuous for 1 h/day on 3 consecutive days

Exposure 1

Main characteristics
Frequency 849 MHz
Type
Exposure duration continuous for 1 h/day on 3 consecutive days
Exposure setup
Exposure source
  • monopole
Chamber temperature controlled 420 mm x 317 mm x 100 mm rectangular cavity exposure chamber
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg - - - -
SAR 10 W/kg - - - -

Reference articles

  • Lee JJ et al. (2008): Acute radio frequency irradiation does not affect cell cycle, cellular migration, and invasion.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The protein expression profiles of the MCF7 cells were not significantly altered following radiofrequency exposure. None of the protein spots on the 2D-DIGE gels showed reproducible changes in three independent experiments. Additionally, there was no alteration in the mRNA and protein levels of three protein spots showing altered expression without reproducibility.
The authors conclude that it seems unlikely that radiofrequency exposure modulates the protein expression profile as they were unable to observe any significant and reproducible changes in the protein expression profiles of the exposed MCF7 cells using high throughput and non-high throughput techniques,

Study character:

Study funded by

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