Primary human umbilical vein endothelial cells (pooled from different donors) and primary human brain microvascular endothelial cells (from a single donor) were used and examined immediately after exposure. 13 independent sham exposed and exposed samples were generated from human umbilical vein endothelial cells and 11 independent sham exposed and exposed samples were generated from human brain microvascular endothelial cells.
|continuous for 1 h
The data showed numerous differences (368 protein spots) between the proteomes of sham exposed human umbilical vein endothelial cells and human brain microvascular endothelial cells. These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone irradiation did not cause any statistically significant changes in protein expression.
In conclusions, exposure of primary human endothelial cells to the mobile phone irradiation at 1800 MHz GSM signal for 1 hour (SAR of 2 W/kg) did not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with earlier studies of the authors showing that the 1800 MHz GSM exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926 (Nylund et al. 2009), as compared with the effect of 900 MHz GSM exposure (Nylund et al. 2006).