Study type: Medical/biological study (experimental study)

Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells. med./bio.

Published in: Proteome Sci 2010; 8 (1): 52

Aim of study (acc. to author)

To study whether exposure to 1800 MHz GSM mobile phone irradiation can affect cell proteome of two types of human primary endothelial cells (primary cell cultures).

Background/further details

Primary human umbilical vein endothelial cells (pooled from different donors) and primary human brain microvascular endothelial cells (from a single donor) were used and examined immediately after exposure. 13 independent sham exposed and exposed samples were generated from human umbilical vein endothelial cells and 11 independent sham exposed and exposed samples were generated from human brain microvascular endothelial cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Modulation type: pulsed
Exposure duration: continuous for 1 h

Exposure 1

Main characteristics
Frequency 1,800 MHz
Type
Exposure duration continuous for 1 h
Modulation
Modulation type pulsed
Additional info

modulation frequencies: 2, 8, 217, 1733 Hz mean duration of 10.8 seconds for non-DTX ("talking") and 5.6 seconds for DTX ("listening")

Exposure setup
Exposure source
Setup The system consisted of two identical exposure chambers mounted inside the same cell culture incubator. One of the chambers acted as a sham exposure and the other as an experimental chamber.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg - measured - -

Reference articles

  • Schuderer J et al. (2004): High Peak SAR Exposure Unit With Tight Exposure and Environmental Control for In Vitro Experiments at 1800 MHz
  • Schönborn F et al. (2001): Basis for optimization of in vitro exposure apparatus for health hazard evaluations of mobile communications.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed numerous differences (368 protein spots) between the proteomes of sham exposed human umbilical vein endothelial cells and human brain microvascular endothelial cells. These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone irradiation did not cause any statistically significant changes in protein expression.
In conclusions, exposure of primary human endothelial cells to the mobile phone irradiation at 1800 MHz GSM signal for 1 hour (SAR of 2 W/kg) did not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with earlier studies of the authors showing that the 1800 MHz GSM exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926 (Nylund et al. 2009), as compared with the effect of 900 MHz GSM exposure (Nylund et al. 2006).

Study character:

Study funded by

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