著者らは以前に、900 MHz GSM携帯電話ばく露がヒト内皮細胞株EA.hy926に多数のタンパク質発現変化を起こすことを示した。今回の実験は、周波数を1800 MHzに変えて同じ細胞株のプロテオームに与える影響を調べた。1800 MHz GSM携帯電話の会話モード模擬信号をSAR＝2.0 W/kgで、温度を37±0.3℃に保って1時間ばく露した。ばく露細胞および擬似ばく露細胞のタンパク質を抽出し、2次元電気泳動（2DE）で分離した結果、擬似ばく露細胞とは異なる発現を示した8つのタンパク質スポットが検出された。そのうち3つは質量分析ではSRG、RP78、PSA1と同定されたが、その先の分析は不首尾であった。また900MHzで影響が見られたタンパク質への影響は見られなかった。
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Investigation of protein expression changes were replicated in ten experiments. Results were compared with the data of an earlier study that used 900 MHz GSM mobile phone irradiation (Nylund and Leszczynski 2004).
Modulation type: pulsed
ばく露時間: continuous for 1 h
|ばく露時間||continuous for 1 h|
|ばく露装置の詳細||two waveguides inside an incubator|
|Sham exposure||A sham exposure was conducted.|
|SAR||2 W/kg||average over time||-||-||-|
Eight of 900 detected proteins were differentially expressed in exposed cells. Three out of these eight proteins were identified using MALDI-MS (spermidine synthase, glucose-regulated protein (GRP78) and proteasome subunit alpha type 1 (PSA1)). Due to the lack of the availability of commercial antibodies the authors were only able to further examine expression of GRP78. Using SDS-PAGE and Western blot method they were not able to confirm the result obtained for GRP78 using two-dimensional electrophoresis. Additionally, no effect of 1800 MHz GSM exposure was found on the protein expression of vimentin and Hsp27. These proteins were affected by the 900 MHz GSM exposure in earlier studies (see "Related articles").
In conclusion, the data suggest that the 900 MHz and 1800 MHz GSM exposures might affect the expression of some proteins in the EA.hy926 cell line. The discrepancy observed here between the expression changes of GRP78 detected with different methods confirms the importance of validation of the results obtained with two-dimensional electrophoresis using other methods, e.g. Western blot.