研究のタイプ: 医学/生物学の研究 (experimental study)

[1800GSM携帯電話電磁界に対するヒト内皮細胞株EA.hy926の応答のプロテオーム解析] med./bio.

Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation.

掲載誌: J Proteomics Bioinform 2009; 2: 455-462

著者らは以前に、900 MHz GSM携帯電話ばく露がヒト内皮細胞株EA.hy926に多数のタンパク質発現変化を起こすことを示した。今回の実験は、周波数を1800 MHzに変えて同じ細胞株プロテオームに与える影響を調べた。1800 MHz GSM携帯電話の会話モード模擬信号をSAR=2.0 W/kgで、温度を37±0.3℃に保って1時間ばく露した。ばく露細胞および擬似ばく露細胞のタンパク質を抽出し、2次元電気泳動(2DE)で分離した結果、擬似ばく露細胞とは異なる発現を示した8つのタンパク質スポットが検出された。そのうち3つは質量分析ではSRG、RP78、PSA1と同定されたが、その先の分析は不首尾であった。また900MHzで影響が見られたタンパク質への影響は見られなかった。

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研究目的(著者による)

To study the effects of 1800 MHz GSM mobile phone irradiation on the protein expression in a human endothelial cell line.

詳細情報

Investigation of protein expression changes were replicated in ten experiments. Results were compared with the data of an earlier study that used 900 MHz GSM mobile phone irradiation (Nylund and Leszczynski 2004).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h
  • SAR: 2 W/kg average over time

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Additional information

dominant modulation components: 2 Hz, 8 Hz, 217 Hz, 1733 Hz GSM talk mode

ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two waveguides inside an incubator
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg average over time - - -

Reference articles

  • Tillmann T et al. (2007): [B6C3F1マウスにおけるGSMおよびDCS無線通信信号の発がん性研究]
  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Eight of 900 detected proteins were differentially expressed in exposed cells. Three out of these eight proteins were identified using MALDI-MS (spermidine synthase, glucose-regulated protein (GRP78) and proteasome subunit alpha type 1 (PSA1)). Due to the lack of the availability of commercial antibodies the authors were only able to further examine expression of GRP78. Using SDS-PAGE and Western blot method they were not able to confirm the result obtained for GRP78 using two-dimensional electrophoresis. Additionally, no effect of 1800 MHz GSM exposure was found on the protein expression of vimentin and Hsp27. These proteins were affected by the 900 MHz GSM exposure in earlier studies (see "Related articles").
In conclusion, the data suggest that the 900 MHz and 1800 MHz GSM exposures might affect the expression of some proteins in the EA.hy926 cell line. The discrepancy observed here between the expression changes of GRP78 detected with different methods confirms the importance of validation of the results obtained with two-dimensional electrophoresis using other methods, e.g. Western blot.

研究の種別:

研究助成

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