High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• molecular biosynthesis: gene expression
• cell viability/cell division/proliferation: cell differentiation, cell cycle phase, and proliferation

Exposure

• • mobile communications
  • GSM

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• molecular biosynthesis: expression of early response and growth regulatory genes (c-fos, c-jun, c-myc, early growth response-1(egr-1), hsp70, p21, p53, bcl-2, alpha-myosin heavy chain (alpha-MHC as degree of cardiac differentiation), beta-tubulin) (RT-PCR)
• cell viability/cell division/proliferation: cell differentiation (cardiac differentiation/number of embryoid body outgrowth containing spontaneously beating cardiomyocytes); cell cycle phase, proliferation (number of cells) (flow cytometry)
• morphol./histopathol. changes: cell morphology

Main outcome of study (acc. to author)

Study funded by

• Fonds der chemischen Industrie (FCI; Foundation of the Chemical Industry), Germany
• VERUM Foundation (Stiftung für Verhalten und Umwelt), Germany

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