animals were housed individually, cages were covered with a stainless steel mesh lid
exposure system consisted of 18 wooden bobbins (0.5 x 0.5 x 0.5 m³); wherein individual bobbins were wound by two sets of horizontal coils (each set of 25 turns); individual turns in the horizontal coil were seperated by a distance of 5 mm; coils were made of 22 gauge enameled copper wire; distance of 0.3 m seperated the individual bobbins to avoid interference
A sham exposure was conducted.
a set-up similar to the exposed animals was build for the sham exposed ones: 18 wooden bobbins were established without energizing the coils (2.78 m between exposed and sham exposed set-up).
神経系への影響: oxidative stress in the brain: lipid peroxidation (level of malondialdehyde via thiobarbituric acid-reactive substances, spectrophotometry), level of reduced glutathione and oxidized glutathione (GSH/GSSG ratio) (fluorescence spectrometry), enzyme activity of superoxide dismutase (spectrophotometry), enzyme activity of glutathione peroxidase (fluorescence spectrometry)
In the hippocampus and the cerebellum of exposed groups (50 µT and 100 µT), the lipid peroxidation and enzyme activity of the glutathione peroxidase were significantly increased compared to the sham exposed group, while in the cortex only the 100 µT-exposed group showed a significant increase. In the hippocampus, cerebellum and cortex, the enzyme activity of the superoxide dismutase was significantly increased in the 100 µT-exposed group in comparison to the sham exposed group, but not in the 50 µT-group. The GSH/GSSG ratio was significantly decreased in all examined brain areas of the 100 µT-exposed group compared to the sham exposure group, while in the 50 µT-exposure group the GSH/GSSG ratio was only significantly decreased in the cerebellum. No significant differences between the groups were seen regarding the body weight. The authors conclude that long-term exposure to a 50 Hz-magnetic field induces oxidative stress in the rat brain.