研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯電話電磁界放射はショウジョウバエのMAPKシグナリングを活性化し、生存を制御する] med./bio.

Mobile phone electromagnetic radiation activates MAPK signaling and regulates viability in Drosophila.

掲載誌: Bioelectromagnetics 2008; 29 (5): 371-379

目的と方法:ショウジョウバエをモデル動物とした実験系で、携帯電話の835MHz電磁界による生物学的影響を調べること。結果:米国規格協会(ANSI)のばく露制限値である比吸収率SAR)値1.6 W/kgにショウジョウバエをばく露させた場合、30時間ばく露の後であっても、その90%が生存していた。しかし、SAR値4.0 W/kgの場合、12時間ばく露から生存率は低下し始めた。電磁界ばく露ストレス反応の引き金となり、活性酸素種の生成を増加させた。また、電磁界ばく露細胞外信号制御キナーゼ(ERK)とc - Jun N末端キナーゼJNK)を活性化させたが、p38キナーゼのシグナリングは活性化されなかった。具体的には、SAR値1.6 W/kgの時には、主にERKシグナリングと抗アポトーシス遺伝子発現が活性化されたが、SAR値4.0W/kgの時には、JNKシグナリングとアポトーシス遺伝子発現が強く活性化された。加えて、SAR値4.0W/kgにおいては、ハエの脳でアポトーシス細胞数が増えた。結論:ショウジョウバエにおいて、ANAIが提唱する電磁界放射ばく露制限値は、ERKの生存シグナリングを引き起こしたが、それより強い電磁界放射はJNKの細胞死シグナリングを活性化した。

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研究目的(著者による)

This study was performed to understand the molecular nature of the biological effects of mobile phone electromagnetic field at the organism level of the fruit fly.

詳細情報

A total of 690 adult female flies, wild type or mutants, were exposed at two different specific absorption rates: at 1.6 W/kg representing the exposure limit proposed by the American National Standards Institute (ANSI) and at 4 W/kg. At least three independent experiments were performed in each electromagnetic field exposure condition. To prove whether decreased viability after exposure is related to changed anti-oxidant status two transgenic mutant flies exhibiting defective superoxide dismutase were crossed (Actin5C-Gal4 and UAS-SOD1IR or UAS-SOD2IR).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835 MHz
ばく露時間: continuous for 6, 12, 18, 24, 30, and 36 h

ばく露1

主たる特性
周波数 835 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 6, 12, 18, 24, 30, and 36 h
ばく露装置
ばく露の発生源/構造
  • patented Wave Exposure V10 device [Chang et al., 2005] based on the TEM wave theory
チャンバの詳細 The exposure device was placed in a chamber maintained at 25 ± 0.1 °C to avoid thermal effects.
ばく露装置の詳細 Flies were exposed in the device in a 100-mm Petri dish with a wet filter paper to maintain humidity.
Additional information At least three independent experiments were performed in each exposure condition.
パラメータ
測定量 種別 Method Mass 備考
SAR 1.6 W/kg - - - -
SAR 4 W/kg - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

At the specific absorption rate 1.6 W/kg, more than 90% of the flies were viable even after the 30 hours exposure. However, at the specific absorption rate 4 W/kg, viability was decreasing from the 12 hours exposure. Only 10% of the flies survived after the 30 hours exposure.
The exposure significantly increased intercellular ROS levels by time and intensity. Superoxide dismutase defect mutants showed lower viability compared to the wild type.
The electromagnetic field exposures activated ERK and JNK signal transduction pathway, but not p38 kinase signal transduction pathway. Interestingly, the specific absorption rate 1.6 W/kg activated mainly ERK signal transduction pathway and expression of an anti-apoptotic gene, whereas the specific absorption rate 4 W/kg strongly activated JNK signal transduction pathway and expression of apoptotic genes.
In addition, the specific absorption rate 4 W/kg amplified the number of apoptotic cells in the fly brain.

研究の種別:

研究助成

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