この研究は、免疫細胞化学と組み合わせた全細胞パッチクランプ分析を用いて、ラットの培養海馬ニューロンの機能に対するマイクロ波ばく露の影響を評価した。2.4 W / kgのSARで、1800-MHz GSMマイクロ波の慢性ばく露（8日間、1日15分）を細胞に与えた。その結果、ばく露により、α-アミノ-3-ヒドロキシ-5-メチル-4-ソキサゾールプロピオン酸（AMPA）型の微小興奮性シナプス後電位（mEPSC）の振幅の選択的減少が引き起こされた；その一方、AMPA mEPSCの周波数およびN-メチル-D-アスパラギン酸（NMDA）mEPSCの振幅は変化しなかった；さらに、GSMばく露により、培養ニューロンのシナプス後肥厚部（PSD）に最も豊富に存在するタンパク質（PSD95）の発現が減少した、と報告している。
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Glutamate receptor channels play a key role in excitatory synaptic transmission. Changes in glutamate receptor function may be involved in the mechanisms of the biological effects of GSM microwaves on brain activity.
One group of glutamate receptors are the ionotropic receptors. These are ligand gated ion channels. Two common glutamate receptors are the NMDA receptor (composed of the subunits NR1 and NR2, which again can have different variants, e.g. NR2A, NR2B) and the AMPA (alpha-amino-3-hydroxy-5-methyl-4-soxazole propionic acid) receptor (composed of the subunits GluR1, GluR2, GluR3, and GluR4) whose agonists include NMDA and AMPA.
In addition, the density of PSD95 was detected in exposed and sham exposed neurons. PSD95, a key component of the postsynaptic density (PSD), aggregates neurotransmitter receptors and downstream enzymes and thus ensures the selective activation of different signal transduction cascades within a single cell.
Cultures of hippocampal neurons were prepared from Sprague-Dawley rats.
Modulation type: pulsed
ばく露時間: repeated daily exposure, 15 min/day, for 8 days
|ばく露時間||repeated daily exposure, 15 min/day, for 8 days|
|Duty cycle||12.5 %|
|Repetition frequency||217 Hz|
|チャンバの詳細||Two rectangular waveguides, one for RF and one for sham exposure, were placed inside a conventional incubator at 37 °C, 5% CO2, and 95% humidity.|
|ばく露装置の詳細||Six 35-mm dishes were positioned in the H field maximum of the standing wave and exposed in E polarisation.|
|Sham exposure||A sham exposure was conducted.|
Chronic exposure to GSM microwaves induced a decrease in the amplitudes of the miniature excitatory postsynaptic currents in AMPA receptors, whereas the amplitudes of NMDA receptors did not change. The frequency of the miniature excitatory postsynaptic currents of the AMPA receptors did not change under exposure.
Chronic exposure did not alter the density of the different receptor subunits.
Chronic microwave exposure induced a decrease in the density of PSD95.
The results indicated that GSM microwaves may reduce excitatory synaptic activity and the number of excitatory synapses in rat hippocampal neurons.