研究のタイプ: 医学/生物学の研究 (experimental study)

[GSM RF電磁界へのばく露はヒト内皮細胞、ラット褐色細胞腫、ラット海馬ニューロンにおけるカルシウムの恒常性に影響しない] med./bio.

Exposure to GSM RF Fields Does Not Affect Calcium Homeostasis in Human Endothelial Cells, Rat Pheocromocytoma Cells or Rat Hippocampal Neurons.

掲載誌: PLoS One 2010; 5 (7): e11828

この研究は、高スループット画像化プラットホームを用いて、900 MHz GSM電磁界SAR: 0.012-2 W/kg)にばく露中の細胞内のCa2+動態をモニターした。ばく露したGSM電磁界は現用の携帯電話機からの放射電磁界を模擬したものである。GSMばく露群、連続波ばく露群、擬似ばく露群のデータを比較した。また、実験した細胞は、ヒト内皮細胞ラット褐色細胞腫細胞株PC12、ラット海馬神経細胞初代培養株である。静止期Ca2+、InsP3受容器作動薬で誘発されるCa2+、Ca2+ATPase阻害剤thapsigarginで誘発したCa2+貯蔵の枯渇とその結果生じるストア作動性Ca2+流入など多様な指標で影響を評価した。その結果、900 MHz GSM電磁界は基本となるCa2+恒常性にも刺激によるCa2+シグナルにも影響しないことが示されたと報告している。

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研究目的(著者による)

To study the putative changes in cellular Ca2+ homeostasis during exposure of cells to 900 MHz GSM fields of differing power in three different cell types.

詳細情報

A novel microscope-based high throughput screening system was applied that allowed the automatic analysis of cells under different exposure regimes.
The experiment consisted of a screening phase and a provocation phase. Within the screening phase all cell types were tested to a 2 W/kg 900 MHz GSM field (30 minutes). If significant changes in Ca2+ level were observed under exposure, additional experiments would be performed at lower SAR values to determine the threshold for the effect. If no significant effects were observed in the screening phase, provocation experiments are undertaken where the Ca2+ homeostasis would be deliberately perturbed: calcium release from intracellular stores was evoked by application of histamine, whilst passive calcium efflux and calcium entry were stimulated by incubation with thapsigargin. For these experiments, the EA.hy926 cells were used and tested at a number of lower SAR values, using both continuous wave and GSM modulation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 30 min.
Screening test with all cell types
  • SAR: 2 W/kg (for all cultures)
  • SAR: 0.25 W/kg (only for PC-12 cells)
  • SAR: 0.5 W/kg (only for PC-12 cells)
  • SAR: 1 W/kg (only for PC-12 cells)
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 30 min.
Provocation test with human endothelial cells
ばく露3: 900 MHz
Modulation type: CW
ばく露時間: continuous for 30 min.
Provocation test with human endothelial cells

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 30 min.
Additional information Screening test with all cell types
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM signal

ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 exposure system consisted of 2 identical TEM cells that were designed to hold a single coverslip each (22 mm diameter) in a Teflon ring sandwich chamber; the bottom conductor of the TEM cell contained hole to allow optical access to the cells for imaging purposes; a personal computer controlled the exposure conditions by setting the amplified power output of the Agilent ESG-D RF Signal Generator to one of the two TEM cells, through an in-line bidirectional coupler and an RF switch; an Agilent EPM-P Power meter measured the incident and reflected power at each TEM cell; samples placed in the center of the TEM cell
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - for all cultures
SAR 0.25 W/kg - - - only for PC-12 cells
SAR 0.5 W/kg - - - only for PC-12 cells
SAR 1 W/kg - - - only for PC-12 cells

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 30 min.
Additional information Provocation test with human endothelial cells
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM signal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.012 W/kg - - - -
SAR 0.062 W/kg - - - -
SAR 0.125 W/kg - - - -
SAR 0.25 W/kg - - - -
SAR 0.5 W/kg - - - -
SAR 1 W/kg - - - -
SAR 2 W/kg - - - -

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 30 min.
Additional information Provocation test with human endothelial cells
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.012 W/kg - - - -
SAR 0.062 W/kg - - - -
SAR 0.125 W/kg - - - -
SAR 0.25 W/kg - - - -
SAR 0.5 W/kg - - - -
SAR 1 W/kg - - - -
SAR 2 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The data indicate that 900 MHz GSM fields did not affect either basal Ca2+ homeostasis or provoked Ca2+ signals. Even at the highest field strengths applied, which exceed typical mobile phone exposure levels, no changes in cellular Ca2+ signals were found.
The authors conclude that under the conditions employed in these experiments, and using a highly-sensitive assay, no consequence of radiofrequency exposure could be detected.

研究の種別:

研究助成

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