研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯電話およびWi-Fiによる乳がん細胞のTRPV1チャネルを介したアポトーシス、酸化ストレスおよび細胞質カルシウム蓄積に対する発生源からの距離の影響の研究] med./bio.

Investigation of the effects of distance from sources on apoptosis, oxidative stress and cytosolic calcium accumulation via TRPV1 channels induced by mobile phones and Wi-Fi in breast cancer cells.

掲載誌: Biochim Biophys Acta Biomembr 2015; 1848 (10) Pt B: 2756-2765

この研究は、ヒト乳がん細胞株MCF-7に、携帯電話またはWi-Fi 装置を用いて電磁界ばく露(1時間)を与え、続いてカプサイシン(CAP刺激を与え、細胞のTRPV1チャネルを介したアポトーシス酸化ストレスなどを測定した。TRPV1はCAP依存性のカルシウムイオン透過性チャネルである。携帯電話またはWi-Fi 装置と細胞との、10、20、25cmとした。その結果、距離0、1、5cmのばく露群では、対照群に比べ、細胞の活性化酸素種産生、カルシウムイオン濃度、アポトーシスなどが高かった;20、25cmでは、対照群と有意差がなかった、と報告している。

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研究目的(著者による)

The effects of exposure of human breast cancer cells to a 900, 1800 or 2450 MHz electromagnetic field on apoptosis, oxidative stress and the cytosolic Ca2+ concentration should be investigated at different distances to the field source.

詳細情報

The cells were divided into 4 groups: 1) control group, exposure to an electromagnetic field of 2) 900 MHz, 3) 1800 MHz and 4) 2450 MHz. In groups 2-4 cells were exposed at distances of 0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm to the exposure source, respectively. Each exposure at each distance was repeated 4-6 times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 hour
  • 電力密度: 12 µW/cm² maximum
  • SAR: 0.36 mW/kg mean (± 0.02 mW/kg)
  • SAR: 0.58 mW/kg maximum
  • SAR: 0.18 mW/kg minimum
ばく露2: 1,800 MHz
ばく露時間: continuous for 1 hour
-
ばく露3: 2,450 MHz
ばく露時間: continuous for 1 hour
-

General information

unit of SAR values not clear (mW/kg or W/kg) - contradictory specifications in text and table 1 and exposure setup and specifications for exposure to 1800 MHz and 2450 MHz fields (fields 2 and 3) not clear

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 hour
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • antenna with falcon tube holder
チャンバの詳細 cells were kept in a circulatory water bath in falcon tubes (6 tubes at a time)
ばく露装置の詳細 the exposure system was located in a special room that was fitted with plastic furniture to minimize the possibility of radiation reflection; the walls of the room were covered by chromium-nickel sheets (thickness: 1 mm) to protect the cells against possible external electromagnetic interference; falcon tubes were placed on a plexi glas table; distance was arranged at 0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm between the falcon tubes and the antenna; temperature in room and flasks was maintained at 37°C and humidity of 83%
パラメータ
測定量 種別 Method Mass 備考
電力密度 12 µW/cm² maximum 測定値 - -
SAR 0.36 mW/kg mean 推定値 - ± 0.02 mW/kg
SAR 0.58 mW/kg maximum 推定値 - -
SAR 0.18 mW/kg minimum 推定値 - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源/構造
  • 詳細不明
ばく露装置の詳細 distance was arranged at 0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm between cells and the antenna
パラメータ

No parameters are specified for this exposure.

ばく露3

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源/構造
  • 詳細不明
ばく露装置の詳細 distance was arranged at 0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm between cells and the antenna
パラメータ

No parameters are specified for this exposure.

Reference articles

  • Kahya MC et al. (2014): [セレンが携帯電話(900 MHz)による乳がん細胞の酸化ストレス、ミトコンドリア機能およびアポトーシスを減少させる]
  • Burkhardt M et al. (1996): [ペトリ皿ばく露装置の数値計算および実験によるドシメトリ]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In all exposure groups (2-4), the cytosolic Ca2+ concentration was significantly increased in samples with a distance of 0-5 cm to the field source compared to the control group.
Cell viability was significantly reduced in group 2 (900 MHz) and 4 (2450 MHz) at distances of 0-5 cm and in group 3 (1800 MHz) at distances of 0-25 cm compared to the control group.
The generation of reactive oxygen species was significantly increased in all exposure groups in samples with a distance of 0-5 cm to the field source in comparison to the control group.
Apoptosis was significantly increased in groups 2 and 3 at distances of 0-10 cm and in group 4 at 0-5 cm compared to the control group.
The enzyme activities of caspase-3 and -9 were significantly increased in all exposure groups in samples with a distance of 0-5 cm to the field source when compared to the control group.
The authors conclude that exposure of human breast cancer cells to 900, 1800 and 2450 MHz electromagnetic fields might increase apoptosis, oxidative stress and the cytosolic Ca2+ concentration at distances of up to 10 cm to the source of the field. Thus, a safety distance of 10 cm to such exposure sources could mean an effective protection against these effects and an administration of TRPV1 ion channel blockers might be a sensible therapeutic approach for their treatment.

研究の種別:

研究助成

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