研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁界放射(Wi-Fi)およびてんかんはラットの海馬および後根神経節においてTRPV1チャネル活性化によるカルシウム流入とアポトーシスを誘導する] med./bio.

Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats.

掲載誌: Metab Brain Dis 2014; 29 (3): 787-799

この研究は、ペンチレンテトラゾール(PTZ)投与により作成したラットてんかん動物モデルの海馬および後根神経節(DRG)における、(TRPV1チャネルを経た)カルシウムイオン流入酸化ストレスアポトーシスに対するWi-Fi(2.45   GHz)ばく露の影響を調べた。ラットを、対照群、PTZ + Wi-Fi群、PTZ + Wi-Fi + カプサゼピン(CPZ:TRPV1阻害薬) に分けた。Wi-Fiの1時間ばく露の後にカプサイシン(CAP刺激を行い、海馬、DRGのニューロンを単離して検査した。その結果、PTZ群では、対照群に比べ、海馬細胞質遊離カルシウムイオン活性酸素種アポトーシスミトコンドリア脱分極などが高くなり、一方、細胞生存率は低下した;Wi-Fiばく露により、細胞質遊離カルシウム上昇の影響はさらに大きくなった;しかし、CPZ投与群では、てんかん誘導性のカルシウムイオン流入酸化ストレスアポトーシスは防止された、と報告している。

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研究目的(著者による)

The effects of exposure of hippocampal and dorsal root ganglion neurons of rats to a 2.45 GHz electromagnetic field (WLAN) on TRPV1 ion channel activity, oxidative stress and apoptosis in association with epilepsy should be investigated.

詳細情報

In the study, two different types of cells were extracted from rats and divided into the following groups: a) groups using hippocampal neurons: 1) from healthy rats (n=10) (control group), 2) from epileptic rats (n=8 or 10?), 3) exposed neurons from epileptic rats (n=8 or 10?), 4) exposed neurons from epileptic rats (n=8 or 10?) and addition of capsazepine.
b) groups using dorsal root ganglion neurons: 5) from healthy rats (n=8) (control group), 6) treatment with capsaicin (from 6 rats), 7) exposed neurons (from 6 rats), 8) exposed neurons (from 6 rats) and addition of capsaicin plus capsazepine after 1 hour, 9) treatment with different calcium channel inhibitors (from 6 rats), 10) exposed neurons and treatment with different calcium channel inhibitors (from 6 rats).
TRPV1 is a calcium-permeable and non-selective ion channel, gated by noxious heat, oxidative stress and capsaicin. To induce epilepsy, pentylenetetrazol was administered to rats. Capsaicin was used to activate TRPV1 ion channels while capsazepine was used as a capsaicin antagonist to block TRPV1.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 1 hour
  • 電力密度: 12 µW/cm² (at the top of the tubes)
  • SAR: 0.52 mW/kg mean (± 0.05 mW/kg; per 1 W incident power)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 hour
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

remark EMF-Portal: The authors also mention a continuous wave (?).

ばく露装置
ばく露の発生源/構造
  • antenna
Distance between exposed object and exposure source 25 cm
チャンバの詳細 exposure system was kept in a specific room which included plastic furniture and the walls of the room were covered by chromium-nickel plates (diameter: 1 mm) for shielding against environmental EMF
ばく露装置の詳細 cells were kept in 15 ml Falcon tubes placed in a non-conductive plexi glass table in a circulatory water bath next to the antenna; temperature in room and in tubes was maintained at 37°C (relative humidity of 83 %)
パラメータ
測定量 種別 Method Mass 備考
電力密度 12 µW/cm² - 測定値 - at the top of the tubes
SAR 0.52 mW/kg mean 計算値 - ± 0.05 mW/kg; per 1 W incident power

Reference articles

  • Jin Z et al. (2012): [HL-60細胞における900 MHz無線周波電磁界とドキソルビシンの組み合わせばく露の影響]
  • Burkhardt M et al. (1996): [ペトリ皿ばく露装置の数値計算および実験によるドシメトリ]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The free intracellular calcium, oxidative stress, apoptosis, mitochondrial membrane depolarization and the enzyme activities of caspase-3 and -9 were significantly increased while cell viability was significantly decreased in epileptic rat hippocampus neurons (group 2) compared to the control group. Additional WLAN exposure (group 3) induced a further intracellular calcium increase, while a treatment of the neurons with capsazepine (group 4) resulted in a protection against epilepsy-induced calcium influx, apoptosis and oxidative stress.
The test on TRPV1 ion channel activity in WLAN exposed and non-exposed dorsal root ganglion neurons and treatment with calcium channel inhibitors (group 10) indicated that WLAN exposure induced calcium influx via the TRPV1 channels.
The authors conclude that exposure of hippocampal and dorsal root ganglion neurons of rats to a 2.45 GHz electromagnetic field (WLAN) and epilepsy could induce a calcium influx in cells via TRPV1 ion channels, oxidative stress and apoptosis and that capsazepine might attenuate these effects.

研究の種別:

研究助成

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