研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットの出生後早期の神経発生に対する短時間電磁放射の影響:FosおよびNADPH-dの組織化学的研究] med./bio.

Effects of short-duration electromagnetic radiation on early postnatal neurogenesis in rats: Fos and NADPH-d histochemical studies.

掲載誌: Acta Histochem 2011; 113 (7): 723-728

この研究は、雌雄のWistarラットの脳室下帯(SVZ)および吻側移動流(RMS)における出生後の神経新生に対する電磁放射(EMR)の全身ばく露の即時的影響を調べた。新生仔ラット(生後7日目:P7)および若年ラット(P28)に、周波数2.45 GHz、平均電力密度2.8 mW / cm2のパルス電磁界EMF)への2時間ばく露を与えた。ばく露後、 FosおよびNADPH-dの免疫組織化学的局在を測定し、神経新生への影響を評価した。その結果、短時間のばく露により、P7およびP28ラットのSVZ細胞選択的に、Fos免疫反応性の上昇が生じた;その一方、ばく露を受けたラットのRMS内には視認できるFos陽性細胞はなかった;これらの知見は、ラットの年齢に関係なく、SVZとRMSの間には、増殖中の細胞にとっての必要条件の違いがあることを示唆する;また、短期間のばく露により、P7ラットのRMS内のNADPH-d陽性細胞に早期成熟が生じた;一方、P28ラットでは、EMRは出生後早期に典型的な形態学的兆候を誘発した、と報告している。

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研究目的(著者による)

To study postnatal neurogenesis in the subventricular zone and rostral migratory stream of rats of both sexes.

詳細情報

At least two regions (the subventricular zone and hippocampal dentate gyrus) of the adult brain are responsible for proliferation and migration of neural precursor cells. The subventricular zone functions as the largest region of neurogenesis in the adult brain. Cells born in the subventricular zone migrate via a restricted pathway, called the rostral migratory stream, to the olfactory bulb where they differentiate into local interneurons.
Newborn postnatal day 7 (P7) and young adult rats (P28) were exposed to pulsed electromagnetic fields (each group n=10).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 h on postnatal day 7 or day 28

General information

For further information on the setup see also: Orendác M, Feník A, Mojzis M, Orendácová J. Biological effects of electromagnetic radiation on living systems with respect to the brain. Psychiatrie 2005 (Suppl.2):83-5.

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h on postnatal day 7 or day 28
Modulation
Modulation type pulsed
ばく露装置
ばく露の発生源/構造
  • 詳細不明
パラメータ
測定量 種別 Method Mass 備考
電力密度 2.8 mW/cm² minimum 測定値 - -
電力密度 6.7 mW/cm² maximum 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that short-term exposure induced increased c-Fos immunoreactivity in the cells of the subventricular zone of P7 and P28 rats. There were no c-Fos positive cells visible within the rostral migratory stream of exposed rats. This finding indicates that probably only the subventricular zone progenitor cells have the complete prerequisites necessary for the c-Fos signal transduction cascade.
Short-term exposure also caused earlier maturation of nitrergic cells (NADPH-diaphorase positive cells) within the rostral migratory stream of P7 rats. The NADPH-diaphorase positive cells appeared several days earlier than in the appropriate controls, and their number and morphology showed characteristics of adult rats. On the other hand, in the young adult P28 rats, electromagnetic field exposure induced morphological signs typical of early postnatal age (i.e. signs of dedifferentiation). These data indicate that electromagnetic field irradiation causes age-related changes in the production of nitric oxide, which may lead to different courses of the proliferation cascade in newborn and young adult neurogenesis.
In conclusion, the data indicate that a single dose of electromagnetic field irradiation represents a stressful event for proliferating cells, and they show that although proliferation runs during the whole of postnatal life, some age-and site-related specificities exist in the subventricular zone and rostral migratory stream neurogenic regions.

研究の種別:

研究助成

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