この研究は、母ラットの妊娠期間中、900MHz電磁界（EMF）ばく露の有無およびメラトニン（MEL）およびオメガ3（ω-3）投与の有無を組合せた条件による実験を実施し、仔ラットの海馬の錐体細胞数を比較した。実験群は、対照、擬似ばく露、EMFのみ、EMF + MEL、MELのみ、EMF + ω-3、ω-3のみの7群とし（各群n=3）、EMF、EMF + MEL、EMF + ω-3は、ばく露用チューブ内で900MHz EMFへの1日60分間のばく露を全妊娠期間、継続した（JEIC注：ばく露レベルは抄録に記述無し）。それぞれの群から自然出産した雄の仔ラットを選び、各条件に対応させて新たな仔ラット群（n=6）を作成し、各群について海馬アンモン角（CA）領域の錐体細胞数を検査した。その結果、EMF群では、対照、擬似ばく露、EMF + MEL、EMF + ω-3に比べ、CA領域の錐体細胞の総数が有意に少なかった；EMF、MEL、ω-3の3群の間に有意差はなかった、と報告している。
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The neurotoxic effects of prenatal exposure of rats to a 900 MHz electromagnetic field on hippocampal cells and the protective effects of melatonin and omega-3 should be investigated.
Pregnant rats were divided into the following groups (n=3 each): 1) exposure to the electromagnetic field, 2) exposure to the electromagnetic field and melatonin injections (50 mg/kg/day), 3) exposure to the electromagnetic field and feeding with omega-3 pellets (10% of food), 4) only melatonin treatment, 5) only omega-3 treatment, 6) sham exposure group, 7) cage control group. After birth, 2 male rats from each mother (total of n=6 per group) were further raised for 28 days without exposure and then investigated.
|ばく露時間||1 hour/day from the 1st to the 21st day of gestation|
|Distance between exposed object and exposure source||1 cm|
|チャンバの詳細||restrainer tubes made of 5 mm thick plexi glass; restrainers consisted of a cylinder (6.5 cm diameter, 12 cm length) and a truncated cone (3 cm length) in which the rat's head was inserted|
|ばく露装置の詳細||6 animals were exposed simultaneously (remark EMF-Portal: but there were only 3 animals per group); during exposure, rats were kept in restrainers and heads were placed in the direction of the antenna for local exposure; the antenna was fixed within a 1 cm distance around the rats' heads; holes were made in the restrainer to allow breathing and to minimize body temperature elevation|
|Sham exposure||A sham exposure was conducted.|
|Additional information||(remark EMF-Portal: groups only treated with melatonin (group 4) or omega-3 (group 5) were probably not sham exposed, i.e., not restrained)|
The total number of neurons in the hippocampus was significantly lower in the exposure group (group 1) compared to all other groups, except for group 5 (omega-3 treatment).
The authors conclude that prenatal exposure of rats to a 900 MHz electromagnetic field might have neurotoxic effects in hippocampal cells and that melatonin and omega-3 might have a protective effect in a co-exposure setting.
(remark EMF-Portal: as only the heads of mothers were exposed to the electromagnetic field and no dosimetry was given for the fetuses, the actual prenatal exposure of the offspring is not comprehensible)