研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスの連想学習に対する電磁パルス(EMP)の影響とメカニズムの予備的研究] med./bio.

Effect of Electromagnetic Pulses (EMP) on associative learning in mice and a preliminary study of mechanism.

掲載誌: Int J Radiat Biol 2011; 87 (12): 1147-1154

本研究は、マウスの連想学習に対する電磁パルス(EMP)の影響を調査し、これらの影響の予備的メカニズムを調べるため、GTEMセルを使ってオスのBALB/cマウスをEMP(ピーク強度400kV/m、立ち上り時間10ns、パルス幅350ns、0.5Hz、総パルス数200個)にばく露させ、マウスの連想学習、脳の酸化ストレス血清生化学的検査及びトコフェロール配糖体(TMG)の防護作用を測定した。その結果、EMPばく露はマウスの連想学習を有意に低下させ、TMGはEMPばく露に対する効果的な防護剤として作用したことから、この影響のメカニズムには、EMPばく露による脳内の酸化ストレスの増加が関係している可能性があることが示されたと、結論している。

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研究目的(著者による)

To investigate the effects of electromagnetic pulses on associative learning in mice (and the protective effect of tocopherol monoglucoside).

詳細情報

Two experiments were performed: One experiment to study the effects on associative learning (with three post exposure times: 12 h, 1 day and 2 day) and another experiment to investigate the biochemical parameters (with six post exposure times: 0, 3, 6, 12, 24, 48 h).
The animals of each experiment were randomly divided into four groups: 1) control group, 2) tocopherol monoglucoside treatment (injected intraperitoneally, 0.6 g/kg), 3) pulse exposure group and 4) tocopherol monoglucoside plus pulse exposure group (eight mice per post exposure time point for each group). All the experiments were repeated three times (total of 24 mice for each post exposure time point in each group).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 500 mHz
Modulation type: pulsed
ばく露時間: ca. 6.5 min

General information

Animals were divided into the following four groups: i) control group ii) treatment with 0.6 g/kg (bodyweight) tocopherol monoglucoside (TMG) iii) exposure to EMF iv) treatment with TMG + exposure to EMF

ばく露1

主たる特性
周波数 500 mHz
タイプ
  • electric field
ばく露時間 ca. 6.5 min
Modulation
Modulation type pulsed
Pulse width 350 ns
Rise time 10 ns
Additional information

200 pulses

ばく露装置
ばく露の発生源/構造
  • GTEMセル
  • spark gap pulse generator
ばく露装置の詳細 500 cm long conical GTEM cell; 16 animals placed in individual exposure holders inside the GTEM cell at one time so, that the major axis of the animal was parallel to the magnetic vector
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 400 kV/m peak - - -
SAR 70 mW/kg average over mass - whole body -

Reference articles

  • Chen YB et al. (2010): [インスリンの生物活性に対する電磁パルス(EMP)の影響およびそのメカニズムに関する予備的研究]
  • Ding GR et al. (2010): [EMPで誘導される密着結合タンパク質発現の変化および血液脳関門の破壊]
  • Li KC et al. (2009): [イン・ビボでのマウスの骨代謝に対する電磁パルスの影響]
  • Ding GR et al. (2009): [ラットの脳微小血管透過性に対する電磁パルスばく露の影響]
  • Wang XW et al. (2008): [電磁パルスばく露がマウスの血液精巣障壁の透過性に与える影響]
  • Li BF et al. (2007): [ラットの血圧に電磁パルスは変動を誘発する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

12 h and one day post exposure associative learning was significantly reduced compared with the sham exposure group, but recovered at two days post exposure.
Compared with the sham exposure group, lipid peroxidation of brain tissue and chemiluminescence intensity increased significantly, while the enzyme activity of the antioxidant enzymes superoxide dismutase, glutathione, glutathione peroxidase, catalase decreased significantly at 3 h, 6 h, 12 h and 1 day post exposure. All these parameters recovered at two day post exposure.
No significant differences between the sham exposure group and the pulse exposed group were found in serum cholesterol and triglycerides.
Pretreatment of mice with tocopherol monoglucoside showed protective effects to pulse exposure.
In conclusion, low frequency pulse exposure significantly decreased associative learning in mice and tocopherol monoglucoside acted as a protective agent from pulse exposure. This mechanism could involve an increase of oxidative stress in the brain by pulse exposure.

研究の種別:

研究助成

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