研究のタイプ: 医学/生物学の研究 (experimental study)

[酵母細胞の出芽に対する静磁界の影響] med./bio.

Effect of static magnetic fields on the budding of yeast cells.

掲載誌: Bioelectromagnetics 2010; 31 (8): 622-629

この研究は、静磁界が単一酵母細胞の出芽に及ぼす影響を、強い磁界(2.93T)および磁場勾配(6100 T2/ m)を発生できる磁気回路を用いて調査した。酵母細胞サッカロマイセス・セレヴィシエを、一様または非一様な静磁界下において、シリカ製毛細管(1辺0.1mmの角形管)入れた含水YPD寒天培地増殖させた。その結果、4時間の磁界ばく露では、出芽酵母細胞の大きさに対する磁界影響はわずかであったが、出芽角度に対しては明らかな影響が見られた;均質磁界の場合、娘酵母細胞の出芽方向は主に磁界方向であった;不均質磁界の場合、娘酵母細胞は磁場勾配が高い方の領域にある毛細管流の軸に沿って出芽する傾向があった、と報告している。

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研究目的(著者による)

To evaluate the effect of static magnetic fields on the budding of single yeast cells.

詳細情報

Yeast cells were exposed in the center of a static magnetic field or in the inner or outer region of an inhomogeneous magnetic field.
Budding in yeast cells is a form of asexual reproduction. This process results in a mother and a smaller daughter cell.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 4 hours
homogenous static magentic field
  • 磁束密度: 2.93 T maximum (at the center of the pole pieces)
ばく露2:
  • DC/static
ばく露時間: continuous for 4 hours
inhomogenous static magentic field
  • magnetic gradient (B x (dB/dx)) of 6100 T²/m (maximum) at the edge of the pole pieces
  • magnetic gradient of 1600 T²/m (minimum) at the edge of the pole pieces
ばく露3:
  • DC/static
ばく露時間: continuous for 4 hours
inhomogenous static magentic field
  • magnetic gradient (B x (dB/dx)) of 6100 T²/m (maximum) at the edge of the pole pieces
  • magnetic gradient of 1600 T²/m (minimum) at the edge of the pole pieces

General information

The growth of yeast cells was monitored at three regions (the center, outside, and edge of the pole pieces), each with a width of 500 µm.

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information homogenous static magentic field
ばく露装置
ばく露の発生源/構造
  • NdFeB magnets
チャンバの詳細 yeast cells were exposed in a square fused-silica capillary in an agar solution that had a 100 x 100 mm² inner section, 300 x 300 mm² outer section, and was 7.5 cm in length; after setting, the capillaries were immediately subjected to the magnetic field experiments, which were conducted under dark conditions at 28.4±0.9 °C
ばく露装置の詳細 magnetic circuit (3 x 4 x 5 cm³) was constructed using two NdFeB magnets, two yokes, two pole pieces, four spacers and two frames; in the center of the magnetic circuit, a pair of iron pole pieces was set with a 400 or 600 µm-gap in a 4 x 4 mm² area; yeast was exposed in the center of the pole pieces
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.93 T maximum 計算値 - at the center of the pole pieces

ばく露2

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information inhomogenous static magentic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
ばく露装置の詳細 yeast was exposed in the edge of the pole pieces
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
参照 - - - - magnetic gradient (B x (dB/dx)) of 6100 T²/m (maximum) at the edge of the pole pieces
参照 - - - - magnetic gradient of 1600 T²/m (minimum) at the edge of the pole pieces

ばく露3

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information inhomogenous static magentic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
ばく露装置の詳細 yeast was exposed outside of the pole pieces
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
参照 - - - - magnetic gradient (B x (dB/dx)) of 6100 T²/m (maximum) at the edge of the pole pieces
参照 - - - - magnetic gradient of 1600 T²/m (minimum) at the edge of the pole pieces

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After exposure to one of the different magnetic field conditions, the growth of budding yeast cells was slightly inhibited compared to unexposed cells. However, the magnetic field exposure clearly influenced the budding angle. After exposure to a homogeneous magnetic field the budding direction of daughter cells was mainly oriented in the direction of the magnetic field. In the inhomogeneous magnetic field, the daughter cells tended to bud along the axis where the magnetic gradient was high. No differences in the budding angle were seen between the inner and outer region of the inhomogeneous field.
The authors conclude that the direction of the magnetic field was the main factor controlling the budding direction of yeast cells.

研究の種別:

研究助成

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