研究のタイプ: 医学/生物学の研究 (experimental study)

[プレインキュベートしたニワトリ胚の脾臓の成長に対するELF磁界の影響] med./bio.

Effects of the ELF-MFs on the development of spleens of preincubated chicken embryos.

掲載誌: Electromagn Biol Med 2013; 32 (3): 301-314

この研究は、ELF磁界ばく露後にインキュベートしたニワトリ胚の脾臓における影響として、病理組織学上・組織形態計測学上・微細構造(透過型電子顕微鏡)上の変化、およびアポトーシス、アルカリフォスファターゼ(ALP)の活性について調べた。無処置の対照群擬似ばく露群、4つのばく露群(磁束密度は、1.33、2.66、5.52、7.32 mT)の6群は全てn=15とし、24時間ばく露擬似ばく露)した後、全ての鶏卵を通常のインキュベート(37℃、湿度60%)し、19日齢の胚を取り出し、ホルマリン固定組織染色し、影響を評価した。その結果、ばく露群では、対照群擬似ばく露群に比べ、胚の脾臓の大きさ減少、脾臓実質うっ血損傷、脾結節数の減少、多形核細胞と洞様毛細血管腔の数の増加、ALP活性レベル上昇、アポトーシス細胞数の増加、核変形やミトコンドリア膨化が見られたと報告している。

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研究目的(著者による)

To investigate the effects of exposure to extremely low frequency magnetic fields on the histology of spleens of chicken embryos.

詳細情報

90 fertilized eggs were divided into six groups (n=15 per group): 1.) control group, 2.) sham exposure, 3.) magnetic field exposure at 1.33 mT, 4.) magnetic field exposure at 2.66 mT, 5.) magnetic field exposure at 5.52 mT and 6.) magnetic field exposure at 7.32 mT. Eggs were exposed or sham exposed for 24 hours. Afterwards, they were incubated for 19 days in the same incubator. At the end of the incubation period, eggs were opened and embryos were analyzed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours
ばく露2: 50 Hz
ばく露時間: continuous for 24 hours
ばく露3: 50 Hz
ばく露時間: continuous for 24 hours
ばく露4: 50 Hz
ばく露時間: continuous for 24 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 coil (length 42 cm, diameter in the inside 19.6 cm, diameter in the outside 11.5 cm) was made of 980 turns of 2.5 mm diameter enameled copperwires; coil chamber was air conditioned; temperature in incubator 37°C; humidity in incubator 60 %; 5 eggs were placed horizontally in the coil in east-west direction; magnetic flow was parallel to the long axis of the embryo's body and perpendicular to the long axis of the eggs
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.33 mT - 測定値および計算値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.66 mT - 測定値および計算値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5.52 mT - 測定値および計算値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 7.32 mT - 測定値および計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • spleen
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the exposed groups (2.66 mT, 5.52 mT, 7,23 mT), the mean length and width of the spleen was significantly decreased compared to the control group and the sham exposure group. While embryos from the control group and the sham exposure group appeared normal, in the exposure groups increased blood flow, damages in the spleen parenchyma, decreases in the number of splenic nodules, increases in the number of granulocytes and sinusoidal spaces of spleens were found.
Electron microscopy revealed deformed nuclei and swollen mitochondria in the exposure group in comparison to the control and the sham exposure group. The number of apoptotic cells was significantly increased in the exposure group compared to the control and the sham exposure. In all exposure groups, the enzyme activity of the alkaline phosphatase was significantly enhanced compared to the control and the sham exposure. Furthermore, the enzyme activity increased with higher magnetic flux densities.
The authors conclude that exposure to the extremely low frequency magnetic field could affect the normal development of the spleen in a chicken embryo.

研究の種別:

研究助成

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