The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary?
Then please contact us →
研究目的（著者による）
To investigate the effects of exposure to extremely low frequency magnetic fields on the histology of spleens of chicken embryos.
詳細情報
90 fertilized eggs were divided into six groups (n=15 per group): 1.) control group, 2.) sham exposure, 3.) magnetic field exposure at 1.33 mT, 4.) magnetic field exposure at 2.66 mT, 5.) magnetic field exposure at 5.52 mT and 6.) magnetic field exposure at 7.32 mT. Eggs were exposed or sham exposed for 24 hours. Afterwards, they were incubated for 19 days in the same incubator. At the end of the incubation period, eggs were opened and embryos were analyzed.
coil (length 42 cm, diameter in the inside 19.6 cm, diameter in the outside 11.5 cm) was made of 980 turns of 2.5 mm diameter enameled copperwires; coil chamber was air conditioned; temperature in incubator 37°C; humidity in incubator 60 %; 5 eggs were placed horizontally in the coil in east-west direction; magnetic flow was parallel to the long axis of the embryo's body and perpendicular to the long axis of the eggs
細胞生存力／細胞分裂/増殖: apoptosis in the spleen (only in the group that was exposed at 7.32 mT) (TUNEL assay, light microscopy)
胚／胎児への影響: number of living and dead embryos (presence of heartbeat); morphological analysis of the whole embryo
形態学/組織学的変化: histopathological analysis of the spleen (hematoxylin-eosin stain, light microscopy); ultrastructural analysis of the spleen (only in the group that was exposed at 7.32 mT) (electron microscopy)
enzyme activity of alkaline phosphatase (spectrophotometry); total protein content (Folin-Ciocalteu reagent, spectroscopy)
In the exposed groups (2.66 mT, 5.52 mT, 7,23 mT), the mean length and width of the spleen was significantly decreased compared to the control group and the sham exposure group. While embryos from the control group and the sham exposure group appeared normal, in the exposure groups increased blood flow, damages in the spleen parenchyma, decreases in the number of splenic nodules, increases in the number of granulocytes and sinusoidal spaces of spleens were found. Electron microscopy revealed deformed nuclei and swollen mitochondria in the exposure group in comparison to the control and the sham exposure group. The number of apoptotic cells was significantly increased in the exposure group compared to the control and the sham exposure. In all exposure groups, the enzyme activity of the alkaline phosphatase was significantly enhanced compared to the control and the sham exposure. Furthermore, the enzyme activity increased with higher magnetic flux densities. The authors conclude that exposure to the extremely low frequency magnetic field could affect the normal development of the spleen in a chicken embryo.
