研究のタイプ: 医学/生物学の研究 (experimental study)

[プレインキュベートしたニワトリ胚心筋のアポトーシスに対する異なるレベルの50 Hz超低周波正弦波磁界の影響] med./bio.

Effects of 50 Hz extremely low frequency sinusoidal magnetic fields on the apoptosis of the hearts of preincubated chicken embryos at different levels of developments.

掲載誌: Int J Radiat Biol 2013; 89 (4): 234-242

この研究は、50Hz正弦波超低周波(ELF)磁界がプレインキュベートしたニワトリ胚の心臓に及ぼす影響を、組織病理学的・生化学的・超微小構造レベルで調べたものである。90個の正常な有精卵を、対照群、偽ばく露群、ばく露群(磁束密度1.33、2.66、5.52、7.32mT)の6群に分けた。ばく露群を、インキュベート前にそれぞれの磁束密度で24時間ばく露した。その後、6群全てを14日間インキュベートした。その結果、1.33mTばく露群では筋細胞損傷;2.66mTばく露群では細胞内空間の増加、核の暗色化およびアポトーシス細胞の増加;5.52及び7.32mTばく露群では軽い炎症筋細胞壊死;7.32mTばく露群では核膜孔の増加、ミトコンドリア内膜での小型円形の不明瞭な襞の増加、筋細胞ミトコンドリア減少、アルカリホスファターゼ活性の上昇が生じることが示された、と報告している。

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研究目的(著者による)

To study the effects of 50 Hz sinusoidal extremely low frequency magnetic fields on preincubated chicken embryo hearts at histopathological, biochemical, and ultrastructural levels.

詳細情報

90 fertilized eggs were divided into 6 groups (each group n=15): control group, sham exposure and four exposure groups exposed to different magnetic flux intensities (24 h before incubation). The hearts were removed on day 14 of embryonic development.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 h

General information

Fertilized eggs were divided into the following six groups: 1) exposure to 1.33 mT 2) exposure to 2.66 mT 3) exposure to 5.52 mT 4) exposure to 7.32 mT 5) control group 6) sham exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 42 cm long cylindrical coil with an inner diameter of 9.6 cm, an outer diameter of 11.5 cm and 980 turns of 2.5 mm enameled copper wire; five eggs placed horizontally in east-west direction in the coil; magnetic flux parallel to the long axis of the embryo's body; exposure chamber ventilated
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.33 mT - 測定値 - group 1
磁束密度 2.66 mT - 測定値 - group 2
磁束密度 5.52 mT - 測定値 - group 3
磁束密度 7.32 mT - 測定値 - group 4

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Histomorphometric investigations of the lengths, widths, thickness of the septae, and left and right ventricles showed normal cells in the hearts of embryos of the control group and sham exposed group in comparison to those of the exposed groups: There was a significant decrease in the lengths of the hearts of exposure group 2 (2.66 mT) and a significant increase in the lengths of the hearts of exposed groups 3 (5.52 mT) and 4 (7.32 mT). Additionally, a significant decrease and increase were observed in the widths of the hearts of exposure group 2 and exposure groups 3 and 4, respectively, The thickness of septae of the hearts of exposed embryos of group 2 showed a significant decrease. There was a significant decrease in the thickness of the wall of the left ventricle in embryos of exposure group 2 and a significant increase in those of exposure groups 1, 3, and 4. The thickness of the wall of the right ventricles was significantly decreased in exposure group 2 while it had increased in the hearts of groups 1, 3, and 4. (All parameters in comparison with sham exposed and control group).
Extremely low frequency magnetic field exposure caused damaged myocytes in exposure group 1, condensed dark nuclei, an increase in the number of apoptotic cells in addition to increased intracellular spaces in exposure group 2, light inflammation and myocyte necrosis in exposure groups 3 and 4, an increase in the number of nuclear membranes' pores, small round unclear cristae mitochondria, and a decrease in the number of mitochondria in the myocytes of exposure group 4 as well as an increase in alkaline phosphatase activities in all exposure groups.
The data provided evidence that extremely low frequency magnetic fields damaged the hearts of chicken embryos at histological, cellular, biochemical level.

研究の種別:

研究助成

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