研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯電話放射はマウスの精母細胞由来の細胞株にモード依存性のDNA損傷を誘発する:メラトニンの防護的役割] med./bio.

Mobile phone radiation induces mode-dependent DNA damage in a mouse spermatocyte-derived cell line: a protective role of melatonin.

掲載誌: Int J Radiat Biol 2013; 89 (11): 993-1001

この研究は、携帯電話電磁界によるマウスの精母細胞由来細胞株GC-2のDNA損傷をアルカリコメットアッセイにより調べた。ばく露は、市販のGSM900MHz携帯電話培養皿の下に置いて行い、待機モード、通話(listen)モード、呼び出中(dialing)モード、呼び出しを受ける(dialed)モードについて実験した。各モードでの放射電力は事前に測定したが、SARについては、人体頭部SARが0.585 W/kg、人の全身SARが0.813 W/kgとの記述があるのみである。対照群、各モードばく露群(各モードで20分に1回1分間のばく露を24時間断続した)、ポジティブコントロール群(メチルメタンスルホネート投与)の他、dialing中モードばく露群でのばく露前のメラトニンの投与群、非投与群について、ばく露後のDNA損傷を比較した。その結果、listen、dialed、dialingモードばく露群でDNA損傷レベルが有意に上昇した;この3者間では、listenよりdialedおよびdialingモードの方が、DNA損傷レベル上昇が有意に高かった;これは放射電力強度の測定値の大きさの順番と一致していた;メラトニンの事前投与群ではこの影響が小さくなった、などを報告している。

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研究目的(著者による)

To examine whether exposure to electromagnetic fields of mobile phones can induce DNA damage in male germ cells of mice and to evaluate the potential protective effect of melatonin.

詳細情報

The following groups of cell cultures were examined: 1.) sham exposure, 2.) mobile phone exposure in "standby mode", 3.) mobile phone exposure "listening mode", 4.) mobile phone exposure "dialed mode", 5.) mobile phone exposure "dialing mode", 6.) mobile phone exposure "dialing mode" + 200 nM melatonin and 7.) positive control (addition of 0.5 mM methyl methanesulfonate, a carcinogen).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
Modulation type: pulsed
standby mode
  • 電力密度: 0.008 µW/cm² (±0.005)
  • 磁束密度: 0.0007 µT mean (±0.0001 (for the 217 Hz component))
  • 磁束密度: 0.001 µT maximum (±0.0002 (for the 217 Hz component))
  • SAR: 0.813 W/kg maximum (whole body) (according to manufacturer's instructions)
  • SAR: 0.585 W/kg maximum (partial body) (according to manufacturer's instruction (highest value for the human head))
ばく露2:
Modulation type: pulsed
ばく露時間: continuous for 1 min every 20 min for 24 h
listening mode
  • 電力密度: 5.9 µW/cm² (±1.16)
  • 磁束密度: 4.159 µT mean (±0.627 (for the 217 Hz component))
  • 磁束密度: 32.853 µT maximum (±4.95 (for the 217 Hz component))
  • SAR: 0.813 W/kg maximum (whole body) (according to manufacturer's instructions)
  • SAR: 0.585 W/kg maximum (partial body) (according to manufacturer's instruction (highest value for the human head))
ばく露3:
Modulation type: pulsed
ばく露時間: continuous for 1 min every 20 min for 24 h
dialed mode
  • 電力密度: 11.69 µW/cm² (±2,17)
  • 磁束密度: 7.631 µT mean (±1.225 (for the 217 Hz component))
  • 磁束密度: 60.728 µT maximum (±8.874 (for the 217 Hz component))
  • SAR: 0.813 W/kg maximum (whole body) (according to manufacturer's instructions)
  • SAR: 0.585 W/kg maximum (partial body) (according to manufacturer's instruction (highest value for the human head))
ばく露4:
Modulation type: pulsed
ばく露時間: continuous for 1 min every 20 min for 24 h
dialed mode
  • 電力密度: 12.2 µW/cm² (±1,84)
  • 磁束密度: 7.611 µT mean (±0.667 (for the 217 Hz component))
  • 磁束密度: 62.407 µT maximum (±5.466 (for the 217 Hz component))
  • SAR: 0.813 W/kg maximum (whole body) (according to manufacturer's instructions)
  • SAR: 0.585 W/kg maximum (partial body) (according to manufacturer's instruction (highest value for the human head))

ばく露1

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
Additional information standby mode
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
  • Motorola XT300
ばく露装置の詳細 mobile phone was placed under the Petri dishes at a distance of 50 mm in the CO2 incubator with its front side facing up towards the bottom of the dish; dishes were located in the middle of the phone at the vertical axis
Sham exposure A sham exposure was conducted.
Additional information cell phone switched on without any active calls
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.008 µW/cm² - 測定値および計算値 - ±0.005
磁束密度 0.0007 µT mean 測定値 - ±0.0001 (for the 217 Hz component)
磁束密度 0.001 µT maximum 測定値 - ±0.0002 (for the 217 Hz component)
SAR 0.813 W/kg maximum - whole body according to manufacturer's instructions
SAR 0.585 W/kg maximum - partial body according to manufacturer's instruction (highest value for the human head)

ばく露2

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
ばく露時間 continuous for 1 min every 20 min for 24 h
Additional information listening mode
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
  • セルラ-電話
  • Motorola XT300
Sham exposure A sham exposure was conducted.
Additional information a call was receveid (playing music), automatically accepted and ended automatically after 1 min.
パラメータ
測定量 種別 Method Mass 備考
電力密度 5.9 µW/cm² - 測定値および計算値 - ±1.16
磁束密度 4.159 µT mean 測定値 - ±0.627 (for the 217 Hz component)
磁束密度 32.853 µT maximum 測定値 - ±4.95 (for the 217 Hz component)
SAR 0.813 W/kg maximum - whole body according to manufacturer's instructions
SAR 0.585 W/kg maximum - partial body according to manufacturer's instruction (highest value for the human head)

ばく露3

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
ばく露時間 continuous for 1 min every 20 min for 24 h
Additional information dialed mode
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
  • セルラ-電話
  • Motorola XT300
Sham exposure A sham exposure was conducted.
Additional information a call was not accepted and ended automatically after 1 min
パラメータ
測定量 種別 Method Mass 備考
電力密度 11.69 µW/cm² - 測定値および計算値 - ±2,17
磁束密度 7.631 µT mean 測定値 - ±1.225 (for the 217 Hz component)
磁束密度 60.728 µT maximum 測定値 - ±8.874 (for the 217 Hz component)
SAR 0.813 W/kg maximum - whole body according to manufacturer's instructions
SAR 0.585 W/kg maximum - partial body according to manufacturer's instruction (highest value for the human head)

ばく露4

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
ばく露時間 continuous for 1 min every 20 min for 24 h
Additional information dialed mode
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
  • セルラ-電話
  • Motorola XT300
Sham exposure A sham exposure was conducted.
Additional information outgoing call, but not accepted by the dialed phone for 1 min
パラメータ
測定量 種別 Method Mass 備考
電力密度 12.2 µW/cm² - 測定値および計算値 - ±1,84
磁束密度 7.611 µT mean 測定値 - ±0.667 (for the 217 Hz component)
磁束密度 62.407 µT maximum 測定値 - ±5.466 (for the 217 Hz component)
SAR 0.813 W/kg maximum - whole body according to manufacturer's instructions
SAR 0.585 W/kg maximum - partial body according to manufacturer's instruction (highest value for the human head)

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The DNA damage was significantly increased in the mobile phone exposed cell cultures (group 3, 4, 5) compared to the sham exposure and the standby mode. Additionally, the DNA damage in the dialed- and dialing mode-exposed cell cultures was significantly higher than in the listen mode-exposed cell cultures. A treatment with melatonin prevented the exposure-related effects.
The data indicate that exposure to electromagnetic fields of mobile phones could induce DNA damage in male germ cells of mice and melatonin could have a protective effect.

研究の種別:

研究助成

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