研究のタイプ: 医学/生物学の研究 (experimental study)

[マウス精母細胞由来細胞株に1800MHz無線周波電磁放射ばく露が引き起こすDNA塩基の酸化的損傷] med./bio.

Exposure to 1800MHz Radiofrequency Electromagnetic Radiation Induces Oxidative DNA Base Damage in a Mouse Spermatocyte-Derived Cell Line.

掲載誌: Toxicol Lett 2013; 218 (1): 2-9

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研究目的(著者による)

To examine whether exposure to radiofrequency electromagnetic fields causes DNA damage in male murine germ cells.

詳細情報

Two experiments were performed. In the first experiment, the dose-related effects were examined: 1.) negative control, 2.) positive control (addition of H2O2), 3.) exposure at specific absorption rate 1 W/kg, 4.) specific absorption rate 2 W/kg and 5.) specific absorption rate 4 W/kg. In the second experiment, the role of oxidative stress was determined: 1.) sham exposure, 2.) sham exposure + alpha-tocopherol, 3.) radiofrequency exposure (4 W/kg) and 4.) radiofrequency exposure (4 W/kg) + alpha-tocopherol.
The experiments were repeated thrice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent (5 min on, 10 min off) for 24 hours
ばく露2: 1,800 MHz
ばく露時間: intermittent (5 min on, 10 min off) for 24 hours
ばく露3: 1,800 MHz
ばく露時間: intermittent (5 min on, 10 min off) for 24 hours

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 24 hours
Modulation
Modulation type pulsed
Additional information

GSM-Talk signal

ばく露装置
ばく露の発生源/構造
チャンバの詳細 cells exposed in Petri dishes; 6 Petri dishes simultaneously
ばく露装置の詳細 two rectangular waveguides; one waveguide used for exposures, other waveguide used for sham exposures; both waveguides were placed inside a CO2 incubator
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg - 測定値 - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - 測定値 - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - 測定値 - -

Reference articles

  • Franzellitti S et al. (2010): [高周波電磁界(GSM 1.8 GHz)から生じるヒト栄養膜細胞株HTR-8/SVNeoにおける過渡的DNA損傷のアルカリ・コメットアッセイでの評価]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The alkaline comet assay showed no significant DNA damage in any of the groups. However, using the modified comet assay, a significant DNA damage in the 4 W/kg (but not at 1 W/kg and 2 W/kg) exposed cell cultures was found in comparison to the sham exposed cell cultures. The mean fluorescence intensity of 8-oxoguanine was significantly higher in the 4 W/kg (but not at 1 W/kg and 2 W/kg) exposed cultures than in the sham exposed cultures. In the 4 W/kg and 2 W/kg (but not at 1/W/kg) exposed cultures, the level of reactive oxygen species was significantly increased compared to the sham exposed cell cultures. All the effects were blocked by an addition of alpha-tocopherol (only tested with a specific absorption rate of 4 W/kg).
The authors conclude that exposure to radiofrequency electromagnetic fields causes a DNA damage in male germ cells via oxidative stress.

研究の種別:

研究助成

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