研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットの熱性けいれんモデルにおける熱性けいれんの潜時、持続時間、および脳波記録に対する磁界ばく露の影響研究] med./bio.

Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model.

掲載誌: Turk J Med Sci 2014; 44 (2): 295-304

この研究は、ラットの熱性けいれん潜時持続時間、および脳波(EEG)記録に対する磁界MFばく露の影響を調べた。ラットは6群(各群n=6)に分けた:擬似ばく露(S)群、熱性けいれん(FC)群、MFばく露MF)群、MF+FC群、FC+MF群、MF+FC+MF群(処置順に表記)。熱性けいれん誘発後に、直腸温潜時持続時間、および脳波を測定した。その結果、繰り返しの高温ばく露により、発作潜時持続時間は減少した;MFばく露は、潜時よりも持続時間の減少により大きく影響した;一方、10または12日間のMFばく露発作潜時を増加させた;MFばく露により、シータ波デルタ波の増加、ベータ波の減少が見られた、と報告している。

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研究目的(著者による)

The effects of exposure of rats to a 50 Hz magnetic field on latency and duration of febrile (=fever) convulsions and the EEG should be investigated.

詳細情報

36 rats were randomly divided into the following groups: 1) no febrile convulsion inductions and sham exposure, 2) febrile convulsion inductions and sham exposure, 3) exposure to magnetic field without febrile convulsion inductions, 4) daily exposure to magnetic field before febrile convulsion inductions, 5) daily exposure to magnetic field after febrile convulsion inductions, 6) daily exposure to magnetic field before and after ffebrile convulsion inductions.
All rats were operatively equipped with electrodes placed directly on the dura mater (the outermost membrane of the meninges) for recording of the EEG. Exposure to the magnetic field and inductions of febrile convulsions started 22 days after the surgery and were conducted for a period of 20 days. Febrile convulsions were induced every second day (total of 10 seizures) by placing the animals of febrile convulsion groups in water with a temperature of 45°C, whereas groups without febrile convulsion were placed in water with 37°C. Depending on the group, this was done before (group 4) or after (group 5) exposure of the respective day or in the meantime (group 6) (remark EMF-Portal: no further information in the article).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: intermittent for 30 minutes every 15 minutes for 540 minutes/day for 20 days (remark EMF-Portal: specifications in the article are unclear)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent for 30 minutes every 15 minutes for 540 minutes/day for 20 days (remark EMF-Portal: specifications in the article are unclear)
ばく露装置
ばく露の発生源/構造
チャンバの詳細 plexiglas cage (40 Œ 17 Œ 13 cm)
ばく露装置の詳細 solenoid was 500 mm in length and 210 mm in diameter, was made of 1400 turns of an insulated soft copper wire with 1.4 mm in diameter and was placed on a fiber base; it was always kept in a north-south direction and its temperature was maintained at 25 ± 2°C
Sham exposure A sham exposure was conducted.
Additional information all devices that could affect the magnetic field were kept away from the experimental area
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT - 測定値および計算値 - -

Reference articles

  • Gulturk S et al. (2010): [ストレプトゾトシンで糖尿病を誘発したラットにおける血液脳関門の透過性に対するインスリンあり/なしでの50Hz磁界の影響]
  • Kavak S et al. (2009): [反復的な50Hzパルス電磁界はラットの胸部大動脈リングの弛緩反応における糖尿病による障害を改善する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The rectal temperature was significantly higher after a febrile seizure than before in all groups with febrile convulsion inductions (2, 4, 5, 6).
The latency duration of seizures was significantly increased in all febrile convulsion groups exposed to a magnetic field (groups 4, 5, 6) compared to group 2 (febrile convulsion inductions without exposure) after 10 and 12 days of exposure.
The seizure duration was significantly higher in group 2 after 2 days of exposure until the end of the experiment compared to groups 4, 5, 6.
The power of alpha waves was significantly increased and the power of beta waves, which were characterized as physiological waves of the waking state, significantly decreased in groups 2-6 compared to group 1 (no febrile convulsion inductions and sham exposure). The power of theta waves and delta waves, which were characterized as pathological waves, was significantly increased in groups 4, 5 and 6 compared to group 1.
The authors conclude that exposure of rats to a 50 Hz magnetic field might decrease the duration of febrile convulsions but could also have a negative effect on brain waves.

研究の種別:

研究助成

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