Die Experimente wurde in einem Expositions-System mit zuverlässiger und genauer Temperatur-Kontrolle durchgeführt. Der Gelmobilitäts-Shift-Assay wurde so geeicht, dass ein ungefähr 10%iger Anstieg in der Aktivierung der DNA-Bindungsaktivität des Hitzeschock-Faktors nach einem Temperaturanstieg von 1°C gemessen werden konnte.
The irradiator facility was set up in a temperature-controlled room. It consisted of 10 radial transmission line (RTL) irradiators formed by two parallel metal plates 4.3 cm apart with the bottom aluminium plate providing mechanical support and temperature equalization among flasks. Each RTL irradiator had a central conical antenna that emitted TEM waves radially outward through the cavity that was bounded by an annulus of RF-absorbing foam backed by a perforated aluminium lamina. The design and calibration of these irradiators used in several studies have been described in detail in the reference articles.
Aufbau
Up to 16 T-75 sealed culture flasks each containing 40 ml of medium (to minimize edge effects) were positioned in each of the RTLs around the antenna. The high SAR values were obtained by loading each flask dielectrically with a shim of alumina (Al2O3) ceramic as described previously.
Zusatzinfo
The temperature of the culture medium in the flasks was maintained at 37 ± 0.3°C with the aid of thermocouples on the bottom plate of the RTL that were calibrated periodically using intraflask fibre-optic probes. All exposures were performed after the warm-up cycle of the RTL was complete.
Pickard WF et al.
(2000):
Experimental and numerical determination of SAR distributions within culture flasks in a dielectric loaded radial transmission line
Moros EG et al.
(1999):
The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks
Dawe AS et al.
(2008):
Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
Hirose H et al.
(2007):
Mobile phone base station-emitted radiation does not induce phosphorylation of Hsp27
Chauhan V et al.
(2007):
Analysis of gene expression in two human-derived cell lines exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field
Chauhan V et al.
(2006):
Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field
Whitehead TD et al.
(2006):
Gene expression does not change significantly in C3H 10T(1/2) cells after exposure to 847.74 CDMA or 835.62 FDMA radiofrequency radiation
Chauhan V et al.
(2006):
Analysis of proto-oncogene and heat-shock protein gene expression in human derived cell-lines exposed in vitro to an intermittent 1.9 GHz pulse-modulated radiofrequency field
Vanderwaal RP et al.
(2006):
HSP27 phosphorylation increases after 45°C or 41°C heat shocks but not after non-thermal TDMA or GSM exposures
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