研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトB細胞リンパ芽球細胞におけるドキソルビシン誘導性のDNA損傷及びその修復に対する1.8 GHz無線周波放射(RFR)の影響] med./bio.

Impact of 1.8-GHz radiofrequency radiation (RFR) on DNA damage and repair induced by doxorubicin in human B-cell lymphoblastoid cells.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2010; 695 (1-2): 16-21

この実験研究は、ヒトのB細胞リンパ芽球細胞にドキシルビシン(DOX)投与して生じさせたDNA修復に対する1.8 GHz無線周波放射(RFR、SAR = 2 W/kg)の影響を調べた。RFR ばく露(E)または擬似ばく露(S)は、DOXの投与前2時間ばく露、投与と同時の2時間ばく露、投与後の6、12、18および24時間ばく露の組み合わせを5通り(EEE、ESS、SES、SSE、SSS)、DOX投与量は0、0.05、0.075、0.10、0.15、0.20μg/mlの6通りについて実験した。DOX投与から6、12、18および24時間後に、コメット分析によりDNA損傷を検査し、%tail DNAを指標として損傷の修復を評価した。結果として、RFR単独(DOX投与量0)ではDNA損傷を生じないこと、EEEの場合、DOX投与によるDNA損傷の修復過程にRFRの影響が見られたことを報告している。

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研究目的(著者による)

To study whether 1.8 GHz radiofrequency irradiation (2 W/kg) can influence DNA repair in human B cell lymphoblastoid cells exposed to doxorubicin (at the doses of 0 µg/ml, 0.05 µg/ml, 0.075 µg/ml, 0.10 µg/ml, 0.15 µg/ml and 0.20 µg/ml).

詳細情報

Five types of combinative exposures of radiofrequency plus doxorubicin were tested: 1) radiofrequency exposure (2 h) before, during and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 2) radiofrequency exposure before and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 3) radiofrequency exposure during and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 4) radiofrequency exposure for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 5) sham exposure before, during and after doxorubicin exposure.
DNA damage was detected at 0 h, 6 h, 12 h, 18 h and 24 h after exposure to doxorubicin.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: continuous for up to 28 h

General information

Cells were treated in five groups: i) EMF exposure before (2 h), during (2h) and after (6 h, 12 h, 18 hr, 24 h) exposure to DOX ii) EMF exposure before (2 h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX iii) EMF exposure during (2h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX iv) EMF exposure after (6 h, 12 h, 18 h, 24 h) exposure to DOX vi) Sham exposure before (2 h), during (2 h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for up to 28 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 probes put inside the waveguide which was placed inside an incubator; dishes positioned in the H-field maximum of the standing wave
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - -

Reference articles

  • Zeng Q et al. (2006): [MCF-7細胞での遺伝子およびタンパク質発現に対するGSM1800MHzの無線周波電磁界の影響]
  • Leszczynski D et al. (2004): [携帯電話放射の生物学的影響を特定するための発見科学的アプローチの適用可能性]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that 1) radiofrequency exposure could not directly induce DNA damage of human B cell lymphoblastoid cells; 2) doxorubicin could significantly induce DNA damage of the lymphoblastoid cells with the dose-response relationship, and there were special DNA repair characteristics of DNA damage induced by doxorubicin; 3) combinative exposure (type 1: exposure to radiofrequency for 2 h, then simultaneous exposure to radiofrequency and doxorubicin, and exposure to radiofrequency for 6 h, 12 h, 18 h and 24 h after exposure to doxorubicin) could obviously influence DNA repair at six and twelve hours after exposure to doxorubicin for four doxorubicin doses (0.075 µg/ml, 0.10 µg/ml, 0.15 µg/ml and 0.20 µg/ml).
The authors conclude, that the data indicate that 1.8 GHz radiofrequency exposure (2 W/kg) did not induce DNA damage directly but may influence the DNA repair of the DNA damage induced by doxorubicin to some extent.

研究の種別:

研究助成

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